Growing Turanga, Populus diverisifolia Schrenk seedlings under natural conditions by in vitro method in the zone of Almaty region, Kazakhstan
Mukhametzhanova O. Коzhavekova A. Bukabayeva Z. Мazharzhanоva К. Rvaidarova G. Baigazakova Z. Mukhamadiev N. Sezg A.
2025University of Guilan
Caspian Journal of Environmental Sciences
2025#23Issue 51143 - 1149 pp.
Turanga is one of the most valuable native species growing in Almaty region and plays an important role in this region in maintaining ecological balance and fighting desertification. Taking into consideration increasing threats to the natural habitats of this species and limitations of traditional propagation methods, this study was aimed at developing an efficient protocol for in vitro propagation and adaptation of Turanga seedlings to natural conditions of the Almaty region. In this study, young explants prepared from mature trees in the region were used to establish in vitro cultivation. Optimization of the hormonal composition of the culture medium at different stages of regeneration and rooting was carefully studied, and finally a comprehensive protocol for gradual hardening of seedlings was designed. According to the quantitative results obtained during this study, sterilization treatment with 2% sodium hypochlorite for 15 minutes showed the best results, maintaining 85% of the viability of the explants and keeping the contamination level at 12%. During the regeneration stage, the hormonal combination containing 1.0 mg L-1 BAP and 0.1 mg L-1 NAA showed the highest efficiency (average 5.2 shoots per explant). During the rooting stage, the 0.5 mg L-1 IBA treatment was found as the most effective treatment with an average root length of 4.8 cm. Final evaluation in the natural field after three months clearly showed the survival of 75% of the seedlings and their significant growth in height (16.8 cm), number of leaves (12.5), and stem diameter (3.2 mm). This study has illustrated that in vitro propagation of Turanga together with a principled hardening protocol is a practical and efficient solution for mass production of seedlings adapted to natural conditions of the Almaty region.
adaptation , Almaty region , In vitro cultivation , Reforestation , Turanga
Text of the article Перейти на текст статьи
Department of Forest Resources, Game Management and Fishery, Faculty of Forestry and Land Resources, Kazakh National Agrarian Research University, Almaty, Kazakhstan
Department of Landscape Design and Landscaping, Faculty of Forestry and Land Resources, Kazakh National Agrarian Research University, Almaty, Kazakhstan
Department of Applied Biology, Faculty of Information Technologies and Economics, Alikhan Bokeikhan University, Semey, Kazakhstan
Kazakh Agro-Technical Research University named after S. Seifullin, Institute of Agriculture and Forestry, Astana, Kazakhstan
Kazakh Research Institute of Plant Protection and Quarantine named after Zh. Zhiembayev, Laboratory of Pesticide Toxicology, Almaty, Kazakhstan
Department of Agriculture, School of Veterinary and Agricultural Research, NAO “Shakarim University, Semey, Kazakhstan
Department of Plant Biological Protection, Kazakh Research Institute of Plant Protection and Quarantine named after Zh. Zhiembayev, Almaty, Kazakhstan
Department of Forestry Engineering, Kastamonu University, Graduate School of Natural and Applied Sciences, Kuzeykent, Kastamonu, Turkey
Department of Forest Resources
Department of Landscape Design and Landscaping
Department of Applied Biology
Kazakh Agro-Technical Research University named after S. Seifullin
Kazakh Research Institute of Plant Protection and Quarantine named after Zh. Zhiembayev
Department of Agriculture
Department of Plant Biological Protection
Department of Forestry Engineering
10 лет помогаем публиковать статьи Международный издатель
Книга Публикация научной статьи Волощук 2026 Book Publication of a scientific article 2026