Isolation and LC-QToF Characterization of Secondary Metabolites from an Endemic Plant Artemisia heptapotamica Poljak
Mukatay U. Samy M.N. Avula B. Katragunta K. Kemelbek M. Zhubanova A. Khan I.A. Ross S.A.
April 2023MDPI
Molecules
2023#28Issue 7
Phytochemical investigation of the aerial parts of Artemisia heptapotamica Poljak led to the isolation of ten known compounds, including four alkyl p-coumarates: octadecyl trans-p-coumarate (1), icosy trans-p-coumarate (2), docosyl trans-p-coumarate (3), and tetracosyl trans-p-coumarate (4), one sesquiterpene lactone: santonin (5), four flavonoids; axillarin (6), quercetin 3-O-methyl ether (7), luteolin (8), and quercetin (9), and one phenolic acid derivative: p-coumaric acid (10). The structures of the isolated compounds were identified by various spectroscopic analyses. Additionally, the antimicrobial activity of the total extract and different fractions was screened, and they exhibited no inhibition of the growth of Candida albicans, C. neoformans, Aspergillus fumigatus, methicillin-resistant Staphylococcus aureus (MRS), E. coli, Pseudomonas aeruginosa, Klebsiella pneumonia, and Vancomycin-resistant Enterococci (VRE) at the tested concentrations ranging from 8 to 200 μg/mL. The identification and tentative characterization of the secondary metabolites were conducted using LC-QToF analysis. This method helps in the putative characterization of sesquiterpene lactones, flavonoids, coumarate derivatives, and aliphatic compounds. The developed method identified 43 compounds, of which the majority were sesquiterpene lactones, such as eudesmanolides, germacranolides, and guaianolide derivatives, followed by flavonoids. The proposed LC-QToF method helps develop dereplication strategies and understand the major class of chemicals before proceeding with the isolation of compounds.
aliphatic compounds , Artemisia heptapotamica , Asteraceae , coumarate derivatives , flavonoids , LC-QTpoF , sesquiterpene lactones
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Department of Biology and Biotechnology, Al-Farabi Kazakh National University, Almaty, 050040, Kazakhstan
National Center for Natural Products Research, School of Pharmacy, University of Mississippi, Oxford, 38677, MS, United States
Department of Pharmacognosy, Faculty of Pharmacy, Minia University, Minia, 61519, Egypt
Department of Chemistry and Chemical Technology, Al-Farabi Kazakh National University, Almaty, 050040, Kazakhstan
Department of BioMolecular Science, Division of Phamacognosy, School of Pharmacy, University of Mississippi, Oxford, 38677, MS, United States
Department of Biology and Biotechnology
National Center for Natural Products Research
Department of Pharmacognosy
Department of Chemistry and Chemical Technology
Department of BioMolecular Science
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