Kinetically modelled approach of xanthan production using different carbon sources: A study on molecular weight and rheological properties of xanthan
Mohsin A. Akyliyaevna K.A. Zaman W.Q. Hussain M.H. Mohsin M.Z. Al-Rashed S. Tan X. Tian X. Aida K. Tariq M. Haider M.S. Khan I.M. Niazi S. Zhuang Y. Guo M.
15 December 2021Elsevier B.V.
International Journal of Biological Macromolecules
2021#1931226 - 1236 pp.
The present study emphasizes improving the overall yield, productivity and quality of xanthan by Xanthomonas campestris using different carbon sources via optimizing the fermentation media and kinetic modelling work. After optimization, six carbon sources and one nitrogen source were selected for xanthan production in 5 L bioreactor. Kinetic modelling was applied to assess the experimental fermentation data and to check its influence on scale-up production. In this work, xanthan production reached 40.65 g/L with a growth-associated rate constant (α) of 2.831, and highest specific growth rate (μm) of 0.37/h while using maltose as the sole carbon source. Furthermore, rheological properties were determined, and Herschel-Bulkley model was employed to assess the experimental data. Interestingly, xanthan obtained from sucrose and glucose showed the highest yield stress (τ0) of 12.50 ± 0.31 and 7.17 ± 0.21. Moreover, the highest xanthan molecular weight of 3.53 × 107 and 3.25 × 107 g/mol were also found with sucrose and glucose. At last, the proposed mechanism of sugar metabolism and xanthan biosynthesis pathway were described. Conclusively, maltose appeared as the best carbon source for maximum xanthan production: while sucrose and glucose gave qualitatively best results. In short, this systematically modelled approach maximizes the potential output and provides a solid base for continuous cultivation of xanthan at large-scale production.
Batch-culture fermentation , Kinetic modelling , Xanthan gum
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State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China
Department of Biotechnology, Al-Farabi Kazakh National University, Almaty, 050040, Kazakhstan
Institute of Environmental Sciences and Engineering, School of Civil and Environmental Engineering, National University of Sciences and Technology (NUST), Sector H-12, Islamabad, 44000, Pakistan
Department of Botany and Microbiology, College of Science, King Saud University, P.O 2455, Riyadh, 11451, Saudi Arabia
Division of Advanced Nanomaterials, Suzhou Institute of Nano-tech and Nano-bionics, Chinese Academy of Sciences (CAS), Suzhou, 215123, China
Applied Science Research Institute, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon, 34141, South Korea
Department of Chemical Engineering, University of Gujrat, HH Campus, Gujrat, 50700, Pakistan
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, China
State Key Laboratory of Bioreactor Engineering
Department of Biotechnology
Institute of Environmental Sciences and Engineering
Department of Botany and Microbiology
Division of Advanced Nanomaterials
Applied Science Research Institute
Department of Chemical Engineering
State Key Laboratory of Food Science and Technology
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