A Novel Antimicrobial Metabolite Produced by Paenibacillus apiarius Isolated from Brackish Water of Lake Balkhash in Kazakhstan
Meene A. Herzer C. Schlüter R. Zayadan B. Pukall R. Schumann P. Schauer F. Urich T. Mikolasch A.
August 2022MDPI
Microorganisms
2022#10Issue 8
Four aerobic bacteria with bacteriolytic capabilities were isolated from the brackish water site Strait Uzynaral of Lake Balkhash in Kazakhstan. The morphology and physiology of the bacterial isolates have subsequently been analyzed. Using matrix assisted laser desorption ionization-time of flight mass spectrum and partial 16S rRNA gene sequence analyses, three of the isolates have been identified as Pseudomonas veronii and one as Paenibacillus apiarius. We determined the capability of both species to lyse pre-grown cells of the Gram-negative strains Pseudomonas putida SBUG 24 and Escherichia coli SBUG 13 as well as the Gram-positive strains Micrococcus luteus SBUG 16 and Arthrobacter citreus SBUG 321 on solid media. The bacteriolysis process was analyzed by creating growth curves and electron micrographs of co-cultures with the bacteriolytic isolates and the lysis sensitive strain Arthrobacter citreus SBUG 321 in nutrient-poor liquid media. One metabolite of Paenibacillus apiarius was isolated and structurally characterized by various chemical structure determination methods. It is a novel antibiotic substance.
antibiotic , antimicrobial , bacterivory , Escherichia coli , lipopeptides , Micrococcus luteus , predation , Pseudomonas putida
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Institute of Microbiology, University Greifswald, Felix-Hausdorff-Straße 8, Greifswald, 17487, Germany
Imaging Center of the Department of Biology, University Greifswald, Friedrich-Ludwig-Jahn-Str. 15, Greifswald, 17487, Germany
Department of Biotechnology, Al-Farabi Kazakh National University, 71, al-Farabi Ave, Almaty, 050040, Kazakhstan
Leibniz Institute DSMZ—German Collection of Microorganisms and Cell Cultures, Inhoffenstrasse 7B, Braunschweig, 38124, Germany
Institute of Microbiology
Imaging Center of the Department of Biology
Department of Biotechnology
Leibniz Institute DSMZ—German Collection of Microorganisms and Cell Cultures
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