Phytochemical composition and antioxidant, antimicrobial, and cytotoxic activities of different solvent extracts of Ajania fastigiata (C. Winkl.) Poljakov
Composição fitoquímica e atividades antioxidante, antimicrobiana e citotóxica de diferentes extratos solventes de Ajania fastigiata (C. Winkl.) Poljakov
Mamurova A. Kairanova G. Amertayeva G. Yedilova A. Rakhymzhan A. Kamalova M. Iglikova A. Kiyekbayeva L. Smagulova G. Mukanova U. Mukhametzhan A. Beyatli A. Kaliyev B.
2025Instituto Internacional de Ecologia
Brazilian Journal of Biology
2025#85
This study investigates the phytochemical composition and biological activities of six solvent extracts-hexane, ethyl acetate, and hydroethanolic fractions-from the roots and aerial parts of Ajania fastigiata (C. Winkl.) Poljakov (Asteraceae), collected in the mountain regions of southeastern Kazakhstan. Phytochemical analysis revealed that the aerial ethyl acetate extract contained the highest levels of bioactive metabolites, including phenolic compounds (34.22 ± 1.98 mg GAE/g), flavonoids (2.74%), organic acids (1.91%), and coumarins (0.54%). Antioxidant potential was evaluated using DPPH and ABTS radical-scavenging assays, with values of SC50 = 133.98 ± 12.91 µg/mL (DPPH) and 209.21 ± 3.70 mg Trolox/g (ABTS). The minimum inhibitory concentration (MIC), determined by the broth microdilution method, showed strong antimicrobial activity of the aerial ethyl acetate fraction, particularly against P.aeruginosa (MIC = 9 µg/mL) and E.faecalis (MIC = 39 µg/mL). Cytotoxicity was assessed by the MTT assay on NIH-3T3, K562, and Saos cell lines. The aerial ethyl acetate fraction exhibited selective cytotoxic effects, with IC50 values of 210 µg/mL for K562 and 360 µg/mL for Saos, while showing negligible activity toward normal fibroblasts (IC50 > 2500 µg/mL). These results confirm the pharmacological potential of A.fastigiata as a promising natural source of antioxidant, antimicrobial, and anticancer agents.
Ajania fastigiata , biological activities , medicinal plants , pharmaceutical technology , phytochemicals
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al-Farabi Kazakh National University, Faculty of Biology and Biotechnology, Department of Botany and Agroecology, Almaty, Kazakhstan
al-Farabi Kazakh National University, Faculty of Medicine and Healthcare, Department of Fundamental Medicine, Almaty, Kazakhstan
al-Farabi Kazakh National University, Department of Fundamental Medicine, Course of Nursing, Almaty, Kazakhstan
Asfendiyarov Kazakh National Medical University, Pharmaceutical School, Department of Pharmaceutical Technology, Almaty, Kazakhstan
Asfendiyarov Kazakh National Medical University, School of General Medicine, Department of Pharmacology, Almaty, Kazakhstan
Medical University of Astana, Astana, Kazakhstan
Asfendiyarov Kazakh National Medical University, International Medical Faculty, Almaty, Kazakhstan
University of Health Science, Department of Medicinal and Aromatic Plants, Istanbul, Turkey
Institute of Botany and Phytointroduction, Laboratory of Geobotany, Almaty, Kazakhstan
Kazakh National Women’s Teacher Training University, Department of Geography, Almaty, Kazakhstan
al-Farabi Kazakh National University
al-Farabi Kazakh National University
al-Farabi Kazakh National University
Asfendiyarov Kazakh National Medical University
Asfendiyarov Kazakh National Medical University
Medical University of Astana
Asfendiyarov Kazakh National Medical University
University of Health Science
Institute of Botany and Phytointroduction
Kazakh National Women’s Teacher Training University
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