The soybean GmNFY-B1 transcription factor positively regulates flowering in transgenic Arabidopsis
Mallano A.I. Li W. Tabys D. Chao C. Yang Y. Anwar S. Almas H.I. Nisa Z. Li Y.
February 2021Springer Science and Business Media B.V.
Molecular Biology Reports
2021#48Issue 21589 - 1599 pp.
Nuclear Factor Y (NF-Y) gene family regulates numbers of flowering processes. Two independent transgenic Arabidopsis lines overexpressing (OX) GmNFY-B1 and GmNFYB1-GR (GmNFYB1 fused with the glucocorticoid receptor) were used to investigate the function of NFY-B1 in flowering. Furthermore, GmNFYB1-GR lines were chemically treated with dexamethasone (Dex, synthetic steroid hormone), cycloheximide (Cyc, an inhibitor of protein biosynthesis), and ethanol to examine their effects on different flowering related marker genes. Our results indicated that the transgenic lines produced longer hypocotyl lengths and had fewer numbers of rosette leaves compared to the wild-type and nf-yb1 mutant plants under both long and short-day (LD and SD) conditions. The qRT-PCR assays revealed that transcript levels of all flowering time regulating genes, i.e. SOC, FLC, FT, TSF, LFY, GI2, AGL, and FCA showed higher transcript abundance in lines OX GmNFYB1-GR. However, FT and GI genes showed higher transcript levels under Dex and Dex/Cyc treatments compared to Cyc and ethanol. Additionally, 24 differentially expressed genes were identified and verified through RNA-seq and RT-qPCR in GmNF-YB1-GR lines under Cyc and Dex/Cyc treatments from which 14 genes were up-regulated and 10 were down-regulated. These genes are involved in regulatory functions of circadian rhythm, regulation of flower development in photoperiodic, and GA pathways. The overexpression of GmNF-YB1 and GmNF-YB1-GR promote flowering through the higher expression of flowering-related genes. Further GmNF-YB1 and its attachment with the GR receptor can regulate its target genes under Dex/Cyc treatment and might act as flowering inducer under LD and SD conditions.
Arabidopsis thaliana , CCAAT-box , Flowering time , NF-YB1 , Nuclear factor Y
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Key Laboratory of Soybean Biology in Chinese Ministry of Education, Northeast Agricultural University, Harbin, 150030, China
State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei, 230036, Anhui, China
Department of Biomedical Sciences, Nazarbayev University School of Medicine, Nur-Sultan, 010000, Kazakhstan
School of Life Science and Technology, Harbin Normal University, Harbin, China
Key Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin, China
Institute of Molecular Biology and Biotechnology, The University of Lahore, Lahore, Pakistan
Department of Botany, University of Agriculture, Faisalabad, Pakistan
Key Laboratory of Soybean Biology in Chinese Ministry of Education
State Key Laboratory of Tea Plant Biology and Utilization
Department of Biomedical Sciences
School of Life Science and Technology
Key Laboratory of Soybean Molecular Design Breeding
Institute of Molecular Biology and Biotechnology
Department of Botany
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