miR-221-5p_R-4 regulates internalized trehalose-induced autophagy by targeting NRBF2 in porcine granulosa cells
Lv J. Liu G. Gao H. He Y. Tang X. Wang Z. Sun K. Bayazitova K. Jiang Z.
December 2024Elsevier B.V.
International Journal of Biological Macromolecules
2024#282
Trehalose, as a food and feed additive, can regulate physiological and pathological processes by activating autophagy, yet the molecular mechanism of trehalose dominated a rise in autophagy has not been elucidated. This study investigated the mechanisms of trehalose-induced autophagy in porcine granulosa cells (PGCs). Trehalose was internalized into PGCs by endocytosis and caused a temporary change of the lysosome pH, ultimately inducing autophagy. Furthermore, miRNA-seq analysis of PGCs indicated that trehalose upregulated the expression of miR-221-5p_R-4, which can target nuclear receptor binding factor 2 (NRBF2) to induce autophagy of PGCs. In summary, this study reveals that miR-221-5p_R-4 targeting NRBF2 was involved in the autophagy of PGCs challenged by trehalose, which was taken up by PGCs through endocytosis.
Autophagy , Granulosa cells , miR-221-5p_R-4 , Porcine , Trehalose
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College of Animal Science and Technology, Key Laboratory of Animal Genetic, Breeding and Reproduction in Shaanxi Province, Northwest A&F University, Shaanxi, Yangling, 712100, China
Department of Food Security, Agrotechnological Faculty, M. Kozybayev North Kazakhstan University, Petropavlovsk, 150000, Kazakhstan
College of Animal Science and Technology
Department of Food Security
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