Role of c-miR-21, c-miR-126, Redox Status, and Inflammatory Conditions as Potential Predictors of Vascular Damage in T2DM Patients

López-Armas G.C. Yessenbekova A. González-Castañeda R.E. Arellano-Arteaga K.J. Guerra-Librero A. Ablaikhanova N. Florido J. Escames G. Acuña-Castroviejo D. Rusanova I.
September 2022 MDPI

Antioxidants
2022 #11 Issue 9

The development of type 2 diabetes mellitus (T2DM) vascular complications (VCs) is associated with oxidative stress and chronic inflammation and can result in endothelial dysfunctions. Circulating microRNAs play an important role in epigenetic regulation of the etiology of T2DM. We studied 30 healthy volunteers, 26 T2DM patients with no complications, and 26 T2DM patients with VCs, to look for new biomarkers indicating a risk of developing VCs in T2DM patients. Peripheral blood samples were used to determine redox state, by measuring the endogenous antioxidant defense system (superoxide dismutase, SOD; catalase, CAT; glutathione reductase, GRd; glutathione peroxidase, GPx; and glucose-6-phosphate dehydrogenase, G6DP) and markers of oxidative damage (advanced oxidation protein products, AOPP; lipid peroxidation, LPO). Additionally, inflammatory marker levels (IL-1, IL-6, IL-18, and TNF-α), c-miR-21, and c-miR-126 expression were analyzed. T2DM patients showed the highest oxidative damage with increased GSSG/GSH ratios, LPO, and AOPP levels. In both diabetic groups, we found that diminished SOD activity was accompanied by increased CAT and decreased GRd and G6PD activities. Diabetic patients presented with increased relative expression of c-miR-21 and decreased relative expression of c-miR-126. Overall, c-miR-21, SOD, CAT, and IL-6 had high predictive values for diabetes diagnoses. Finally, our data demonstrated that IL-6 exhibited predictive value for VC development in the studied population. Moreover, c-miR-21 and c-miR-126, along with GPx and AOPP levels, should be considered possible markers for VC development in future studies.

circulating miR-21 , IL-6 , miR-126 , oxidative stress markers , type 2 diabetes mellitus , vascular complications

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Departamento de Investigación y Extensión, Centro de Enseñanza Técnica Industrial, C. Nueva Escocia 1885, Guadalajara, 44638, Mexico
Department of Biophysics, Biomedicine and Neuroscience, Al-Farabi Kazakh National University, Al-Farabi Av. 71, Almaty, 050040, Kazakhstan
Laboratorio de Microscopia de Alta Resolución, Departamento de Neurociencias, Centro Universitario de Ciencias de la Salud (CUCS), Universidad de Guadalajara, Sierra Mojada 950, Guadalajara, 44340, Mexico
División de Medicina Interna, Nuevo Hospital Civil Juan I. Menchaca, Universidad de Guadalajara, Salvador Quevedo y Subieta 750, Guadalajara, 44340, Mexico
Centro de Investigación Biomédica en Red Fragilidad y Envejecimiento Saludable (CIBERFES), Instituto de Investigación Biosanitaria de Granada (Ibs), Granada, 18016, Spain
Centro de Investigación Biomédica, Instituto de Biotecnología, Parque Tecnológico de Ciencias de la Salud, Universidad de Granada, Granada, 18016, Spain
Department of Physiology, Faculty of Medicine, University of Granada, Granada, 18016, Spain
Department of Biochemistry and Molecular Biology I, Faculty of Science, University of Granada, Granada, 18019, Spain

Departamento de Investigación y Extensión
Department of Biophysics
Laboratorio de Microscopia de Alta Resolución
División de Medicina Interna
Centro de Investigación Biomédica en Red Fragilidad y Envejecimiento Saludable (CIBERFES)
Centro de Investigación Biomédica
Department of Physiology
Department of Biochemistry and Molecular Biology I

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