Nuclear genome size analysis in Krascheninnikovia ceratoides s. l. (Amaranthaceae)


Ломоносова М.Н., Панкова Т.В., Королюк Е.А., Шауло Д.А., Осмона- ли Б., Николин Е.Г. Анализ размера генома Krascheninnikovia ceratoides s.l. (Amaranthaceae)
Lomonosova M.N. Pankova T.V. Korolyuk E.A. Shaulo D.N. Osmonali B. Nikolin E.G.
2024BGI FEB RAS

Botanica Pacifica
2024#13Issue 1183 - 187 pp.

The total nuclear DNA amount (2C value) of 75 individuals of Krascheninnikovia ceratoides s. l. was assessed by flow cytometry in 16 populations. The mean 2C value in all samples ranged from 2.59 to 6.99 pg. The relationship between total 2C values and ploidy level was calibrated using chromosome counting in four populations. Diploids (2n = 18) and tetraploids (2n = 36) were revealed. In di­ ploid plants, mean 2C value varied between 2.59 to 3.72 pg, in tetraploids − from 6.04 to 6.99 pg. The GS of diploids is approximately half the GS of tetraploids. Diploids were observed in seven populations from the Orenburg Region and the Republic of Tyva (Russia), Kyzylorda and Almaty Regions (Kazakhstan). Tetra­ ploids were recorded in eight populations from Kazakhstan, Orenburg and No­ vosibirsk Regions, from the Republics of Altai and Sakha (Yakutia). One mixed population was found in the Republic of Khakassia represented by di- and tetra­ ploids. We did not find any regularity in the geographical distribution of the two cytotypes. The studied plants showed no significant morphological features of taxonomic value, therefore we consider all the samples belong to Krascheninnikovia ceratoides s. str.

2C nuclear DNA content , chromosome number , cytotype , flow cytometry , genome size variation , ploidy level

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Central Siberian Botanical Garden SB RAS, Novosibirsk, Russian Federation
Institute of Botany and Phytointroduction, Almaty, Kazakhstan
Institute for Biological Problems of Cryolithozone SB RAS, Yakutsk, Russian Federation

Central Siberian Botanical Garden SB RAS
Institute of Botany and Phytointroduction
Institute for Biological Problems of Cryolithozone SB RAS

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