Evaluation of Histochemical Profile and Numerical Indicators of Salvia dumetorum, Growing in Central Kazakhstan
Levaya Y.K. Atazhanova G.A. Ishmuratova M.Y. Zholdasbayev M.E. Medeshova A.T.
2025Research Journal of Pharmacy and Technology
Research Journal of Pharmacy and Technology
2025#18Issue 126077 - 6083 pp.
Salvia dumetorum (L.) Andrz. ex Besser is a promising medicinal plat used in traditional medicine for treating various diseases. In this study, we continue investigation of S. dumetorum in Central Kazakhstan in terms of histochemical profile and numerical parameters of raw material. Histochemical profile was assessed using histochemical tests of cross sections of leaves, petioles, stems and sepals of S. dumetorum in Strauss-Fleming solution and were examined under light microscope, in order to determine secondary metabolites, such as essential oil, phenolic acids, flavonoids, sesquiterpene lactones, polysaccharides and alkaloids. Numerical parameters were assessed using methods described in State Pharmacopoeia of the Republic of Kazakhstan. Determination of quantitative content of heavy metals was carried out using the inductively coupled plasma atomic emission spectrometry (ICP-AES) method. The aim of the study was to determine histochemical profile and numerical parameters of S. dumetorum raw material. Histochemical analysis of raw material demonstrated that S. dumetorum contain high amount of phenolic compounds, flavonoids and essential oil. However, sesquiterpene lactones, polysaccharides and alkaloids were not detected in raw material. Results of assessing numerical parameters showed that all parameters: foreign impurities 1.58±0.11%, total ash 12±0.22%, ash insoluble in 10% HCl 1.66±0.23%, moisture content 6.5±0.17%, extractives 64.17±0,31% meet the requirements of regulatory documents. For the first time, the localization of secondary metabolites was studied using histochemical tests in the tissues of S. dumetorum growing in Central Kazakhstan. The analysis of raw material quality indicators showed that S. dumetorum match with the requirements of State Pharmacopoeia of the Republic of Kazakhstan. This species has biological activity and significant thickets in nature, successfully grown in culture, which confirms the prospect of its practical use as a medicinal plant. The data obtained will be used to confirm the authenticity, identification and standardization of the S. dumetorum and in the technological process for the production of extraction preparations.
Flavonoids , Medicinal plant raw material , Phenolic compounds , Plant structure , Quality control , Salvia dumetorum , Secondary metabolites
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School of Pharmacy, Karaganda Medical University, Gogol 40, Karaganda, Kazakhstan
Department of Botany, Karaganda Buketov University, Universitetskaya 28, Karaganda, Kazakhstan
School of Pharmacy
Department of Botany
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