Molecular and Cellular Mechanism of Action of Chrysotile Asbestos in MRC5 Cell Line
Kussainova A. Bulgakova O. Aripova A. Ibragimova M. Pulliero A. Begimbetova D. Bersimbaev R. Izzotti A.
November 2023Multidisciplinary Digital Publishing Institute (MDPI)
Journal of Personalized Medicine
2023#13Issue 11
Asbestos is a known carcinogen; however, the influence of chrysotile asbestos on the development of tumor-related diseases remains a subject of intense debate within the scientific community. To analyze the effect of asbestos, we conducted a study using the MRC5 cell line. We were able to demonstrate that chrysotile asbestos stimulated the production of reactive oxygen species (ROS), leading to cell death and DNA damage in the MRC5 cell line, using various techniques such as ROS measurement, comet assay, MTT assay, and qPCR. In addition, we found that chrysotile asbestos treatment significantly increased extracellular mitochondrial DNA levels in the culture medium and induced significant changes in the expression profile of several miRNAs, which was the first of its kind. Thus, our research highlights the importance of studying the effects of chrysotile asbestos on human health and reveals multiple adverse effects of chrysotile asbestos.
cf mtDNA , chrysotile asbestos , lung cancer , microRNA , reactive oxygen species
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Department of Health Sciences, University of Genova, Via Pastore 1, Genoa, 16132, Italy
Department of General Biology and Genomics, Institute of Cell Biology and Biotechnology, L.N. Gumilyov Eurasian National University, Astana, 010008, Kazakhstan
National Laboratory Astana, Nazarbayev University, Astana, 010000, Kazakhstan
Department of Experimental Medicine, University of Genoa, Genoa, 16132, Italy
IRCCS Ospedale Policlinico San Martino, Genoa, 16132, Italy
Department of Health Sciences
Department of General Biology and Genomics
National Laboratory Astana
Department of Experimental Medicine
IRCCS Ospedale Policlinico San Martino
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