Comparative Histochemical Analysis of the Aerial Parts of Fragaria viridis and Fragaria vesca (Rosaceae) Growing in the Central Kazakhstan Region
Kurmantayeva G.K. Ishmuratova M.Yu. Atazhanova G.A. Samorodov A.V. Smagulov M.K. Ramazanova A.
2025Research Journal of Pharmacy and Technology
Research Journal of Pharmacy and Technology
2025#18Issue 104828 - 4832 pp.
Histochemical Analysis of Medicinal Plants and Raw Materials is a method aimed at studying the localization of chemical substances and their metabolic products in plant tissues. This article presents the results of the histochemical analysis of the leaves and petioles of Fragaria viridis and Fragaria vesca. The genus Fragaria belongs to the Rosaceae family, and includes several species known for their high content of biologically active compounds, including phenolic compounds, flavonoids, and lipids. These substances contribute to the medicinal properties of strawberries, making them valuable both in traditional medicine and modern pharmacology. Objective: To determine the characteristics of the localization of specific groups of secondary metabolites in the aerial parts of Fragaria viridis and Fragaria vesca growing in the Central Kazakhstan region at the microscopic level. Materials and Methods: Transverse sections of the leaves and petioles of F.viridis and F.vesca were fixed in Strauss-Fleming solution, after which histochemical reactions were performed to determine the location and identify essentialoils, phenolic acids, flavonoids, sesquiterpene lactones, polysaccharides, and alkaloids. The study of microscopic features and histochemical tests was conducted according to the methods of the State Pharmacopoeia of the Republic of Kazakhstan using a Biomed-4 light microscope. Results: Histochemical methods using light microscopy were employed to determine the localization of biologically active substances in the studied plant raw material. The presence of phenolic acids, flavonoids, and essential oils was detected. The absence of sesquiterpene lactones, polysaccharides, and alkaloids in the examined material was established. Conclusion: For the first time, histochemical tests were used to study the localization of secondary metabolites in the tissues of F. viridis and F. vesca. The results of these histochemical studies can be utilized for confirming authenticity, identification, and standardization of the aerial parts of F. viridis and F. vesca.
Epidermis , Essential oil , Flavonoids , Genus Fragaria , Glands , Histochemical analysis , plant , Plant structure , Secondary metabolites , Strawberry , Trichomes
Text of the article Перейти на текст статьи
NC JSC“Karaganda Medical University”, Gogol str., 40, Karaganda, 100000, Kazakhstan
NCJSC“E.A. BuketovKaraganda University”, Universitetskaya str.,28, Campus 3, Karaganda, 100028, Kazakhstan
Bashkir State Medical University, Lenina, 3, Republic of Bashkortostan, Ufa, Russian Federation
NC JSC“Karaganda Medical University”
NCJSC“E.A. BuketovKaraganda University”
Bashkir State Medical University
10 лет помогаем публиковать статьи Международный издатель
Книга Публикация научной статьи Волощук 2026 Book Publication of a scientific article 2026