Altered Expressions of IL-15, IFNG, and HPRT1 Genes in the Thin Endometria of Patients with Reproductive Disorders: A Prospective Comparative Study


Kurmanova A. Ashirbekov Y. Kurmanova G. Mamedaliyeva N. Anartayeva G. Moshkalova G. Salimbayeva D. Tulesheva A. Zhankina Z.
October 2024Multidisciplinary Digital Publishing Institute (MDPI)

Journal of Clinical Medicine
2024#13Issue 20

Reproductive disorders are common events in modern reproductive medicine, occurring both in spontaneous and assisted pregnancies. Studies on the molecular mechanisms of implantation disorders in thin endometria, including the study of gene transcriptional activities, have shed light on the identification of the potential biological markers of endometrial receptivity. Background/Objectives: The goal of this study was to reveal the significantly dysregulated selected gene expressions between RIF and RPL patients with thin endometria. Methods: Endometrial samples were collected from RIF patients (n = 20) and RPL patients (n = 19) during the implantation window days (LH + 7—LH + 10) of their natural menstrual cycles. Ten genes were chosen as the target genes regarding their possible relations with the implantation process. The total RNA was purified and reverse-transcribed, and gene expressions were quantified by RT-PCR. Results: The expressions of the IL-15, INFG, and HPRT1 genes were significantly decreased in the RIF patients with thin endometria compared to the RPL patients (log2 fold change = 0.92, p = 0.023 for IL-15; log2 fold change = 1.24, p = 0.046 for INFG; and log2 fold change = 0.579, p = 0.046 for HPRT1). There were no significant differences in the expressions of the CXCL8, CXCL1, MMP10, C4BPA, TNC, VEGFB, and HAND2 genes between the groups. Conclusions: Decreased expressions of the IL-15, INFG, and HPRT1 genes were found in patients with RIF with thin endometria compared to the endometria of women with RPL. This has practical significance for clinicians for the differentiated prescription of immunomodulatory therapy in patients undergoing ART programs.

endometrial receptivity , recurrent implantation failure , recurrent pregnancy loss , thin endometrium , transcriptomics

Text of the article Перейти на текст статьи

Faculty of Medicine and Healthcare, Al Farabi Kazakh National University, 71, Al-Farabi Avenue, Almaty, 050040, Kazakhstan
Laboratory of Structural and Functional Genomics, M. Aitkhozhin Institute of Molecular Biology and Biochemistry, 86, Dosmukhamedov Street, Almaty, 050012, Kazakhstan
Department of Science and Strategy, Scientific Center of Obstetrics, Gynecology and Perinatology, 125, Dostyk Ave., Almaty, 050010, Kazakhstan
Faculty of Natural Sciences, Friedrich Alexander University Erlangen Nürnberg, Schlossplatz 4, Erlangen, 91054, Germany

Faculty of Medicine and Healthcare
Laboratory of Structural and Functional Genomics
Department of Science and Strategy
Faculty of Natural Sciences

10 лет помогаем публиковать статьи Международный издатель

Книга Публикация научной статьи Волощук 2026 Book Publication of a scientific article 2026