Active O-acetylserine-(thiol) lyase A and B confer improved selenium resistance and degrade l-Cys and l-SeCys in Arabidopsis
Kurmanbayeva A. Bekturova A. Soltabayeva A. Oshanova D. Nurbekova Z. Srivastava S. Tiwari P. Dubey A.K. Sagi M.
18 April 2022Oxford University Press
Journal of Experimental Botany
2022#73Issue 82525 - 2539 pp.
The roles of cytosolic O-acetylserine-(thiol)-lyase A (OASTLA), chloroplastic OASTLB, and mitochondrial OASTLC in plant selenate resistance were studied in Arabidopsis. Impairment in OASTLA and OASTLB resulted in reduced biomass, chlorophyll and soluble protein content compared with selenate-treated OASTLC-impaired and wild-type plants. The generally lower total selenium (Se), protein-Se, organic-sulfur and protein-sulfur (S) content in oastlA and oastlB compared with wild-type and oastlC leaves indicated that Se accumulation was not the main cause for the stress symptoms in these mutants. Notably, the application of selenate positively induced S-starvation markers and the OASTLs, followed by increased sulfite reductase, sulfite oxidase activities, and increased sulfite and sulfide concentrations. Taken together, our results indicate a futile anabolic S-starvation response that resulted in lower glutathione and increased oxidative stress symptoms in oastlA and oastlB mutants. In-gel assays of l-cysteine and l-seleno-cysteine, desulfhydrase activities revealed that two of the three OASTL activity bands in each of the oastl single mutants were enhanced in response to selenate, whereas the impaired proteins exhibited a missing activity band. The absence of differently migrated activity bands in each of the three oastl mutants indicates that these OASTLs are major components of desulfhydrase activity, degrading l-cysteine and l-seleno-cysteine in Arabidopsis.
Arabidopsis , L-cysteine desulfhydrase , L-seleno-cysteine desulfhydrase , OASTLs , selenium metabolism , sulfur metabolism
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Department Of Biotechnology And Microbiology, L. N. Gumilyov Eurasian National University, Nur-Sultan, Kazakhstan
The Albert Katz International School For Desert Studies, The Jacob Blaustein Institutes For Desert Research, Ben-Gurion University Of The Negev, Sede Boqer Campus, Israel
Jacob Blaustein Center For Scientific Cooperation, The Jacob Blaustein Institutes For Desert Research, Ben-Gurion University Of The Negev, Sede Boqer Campus, Israel
The Albert Katz Department Of Dryland Biotechnologies, French Associates Institute For Agriculture And Biotechnology Of Dryland, The Jacob Blaustein Institutes For Desert Research, Ben-Gurion University Of The Negev, Sede Boqer Campus, Israel
Department Of Biotechnology And Microbiology
The Albert Katz International School For Desert Studies
Jacob Blaustein Center For Scientific Cooperation
The Albert Katz Department Of Dryland Biotechnologies
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