Development of New Cotton Varieties Resistant to Verticillium and Fusarium Wilt

Kurbonov A. Khashımova N. Makhmadjanov S. Kostakov A. Tokhetova L. Mamedova F. Ravshanov A. Turaev O. Babaeva D. Makhadjanov D.
2026 John Wiley and Sons Ltd

International Journal of Agronomy
2026 #2026 Issue 1

Cotton production worldwide is severely threatened by soil-borne fungal pathogens, primarily Verticillium dahliae (VD) and Fusarium oxysporum f. sp. vasinfectum (FOV). These wilts can cause seedling loss and substantial yield reduction, making the development of resilient cultivars essential. This study utilized an integrated breeding approach, combining marker-assisted selection (MAS) with rigorous phytopathological and biochemical analyses, to accelerate the development of wilt-resistant Gossypium hirsutum lines. We screened 10 fungal isolates and 8 complex interlinear hybrids across multiple generations (F₇–F₁₁). Genotyping using six SSR markers, including BNL4003, CM209, and TMB0161, identified the hybrid CBRI_3 as the most promising source of resistance alleles. Biochemical studies confirmed that two hybrids, CBRI_3 and CBRI_8, exhibited superior resistance. In these resistant lines, peroxidase enzyme activity was significantly 1.3–1.6 times higher than that in susceptible controls, promoting vital cell wall reinforcement and lignification, which are key defenses against fungal penetration. This integrated approach successfully led to the isolation of the highly resistant line, L-1077 (derived from CBRI_3). This line maintains high fiber quality (Type IV standard) and superior yield while showing strong resistance to both V. dahliae and FOV. The line L-1077 was submitted for patent registration under the variety designation C-6602 (NAP 2020 0007). Copyright

DNA markers , Fusarium oxysporum , generation , Gossypium hirsutum L. , hybrid , molecular genetics , morphology , pathogenicity , peroxidase , phenotypes , selection , strains , variety , Verticillium dahliae

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Cotton Biotechnology Laboratory, Cotton Breeding, Seed Production and Agrotechnologies Research Institute, Tashkent, Uzbekistan
Enzyme Chemistry Laboratory, Institute of Bioorganic Chemistry Named After O.Sodikov, Tashkent, Uzbekistan
Department of Crop Variety Transfer and Adaptation, Agricultural Experimental Station of Cotton and Melon Growing, Atakent, Kazakhstan
Department of Agricultural Technologies, Korkyt Ata Kyzylorda University, Kyzylorda, Kazakhstan
Biotechnology Laboratory, Research Institute of Plant Genetic Resources, Tashkent, Uzbekistan

Cotton Biotechnology Laboratory
Enzyme Chemistry Laboratory
Department of Crop Variety Transfer and Adaptation
Department of Agricultural Technologies
Biotechnology Laboratory

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