Comparative structural insight into prefoldin subunints of archaea and eukaryotes with special emphasis on unexplored prefoldin of Plasmodium falciparum

Kumar V. Behl A. Shoaib R. Abid M. Shevtsov M. Singh S.
2022 Taylor and Francis Ltd.

Journal of Biomolecular Structure and Dynamics
2022 #40 Issue 8 3804 - 3818 pp.

Prefoldin (PFD) is a heterohexameric molecular chaperone which bind unfolded proteins and subsequently deliver them to a group II chaperonin for correct folding. Although there is structural and functional information available for humans and archaea PFDs, their existence and functions in malaria parasite remains uncharacterized. In the present review, we have collected the available information on prefoldin family members of archaea and humans and attempted to analyze unexplored PFD subunits of Plasmodium falciparum (Pf). Our review enhances the understanding of probable functions, structure and mechanism of substrate binding of Pf prefoldin by comparing with the available information of its homologs in archaea and H. sapiens. Three PfPFD out of six and a Pf prefoldin-like protein are reported to be essential for parasite survival that signifies their importance in malaria parasite biology. Transcriptome analyses suggest that PfPFD subunits are up-regulated at the mRNA level during asexual and sexual stages of parasite life cycle. Our in silico analysis suggested several pivotal proteins like myosin E, cytoskeletal protein (tubulin), merozoite surface protein and ring exported protein 3 as their interacting partners. Based on structural information of archaeal and H. sapiens PFDs, P. falciparum counterparts have been modelled and key interface residues were identified that are critical for oligomerization of PfPFD subunits. We collated information on PFD-substrate binding and PFD-chaperonin interaction in detail to understand the mechanism of substrate delivery in archaea and humans. Overall, our review enables readers to view the PFD family comprehensively. Communicated by Ramaswamy H. Sarma Abbreviations: HSP: Heat shock proteins; CCT: Chaperonin containing TCP-1; PFD: Prefoldin; PFLP: Prefoldin like protein; PfPFD: Plasmodium falciparum prefoldin; Pf: Plasmodium falciparum; H. sapiens: Homo sapiens; M. thermoautotrophicus: Methanobacterium thermoautotrophicus; P. horikoshii: Pyrococcus horikoshii.

actin , chaperonin , gametocytes , Plasmodium falciparum , Prefoldin , tubulin

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Special Centre for Molecular Medicine, Jawaharlal Nehru University, New Delhi, India
Medicinal Chemistry Laboratory, Department of Biosciences, Faculty of Natural Sciences, Jamia Millia Islamia, New Delhi, India
Center for Translational Cancer Research Technische, Universität München (TranslaTUM), Radiation Immuno Oncology group, Klinikum rechts der Isar, Munich, Germany
Institute of Cytology of the Russian Academy of Sciences (RAS), St. Petersburg, Russian Federation
Department of General Surgery, Pavlov First Saint Petersburg State Medical University, Petersburg, Russian Federation
Almazov National Medical Research Centre, Polenov Russian Scientific Research Institute of Neurosurgery, St. Petersburg, Russian Federation
National Center for Neurosurgery, Nur-Sultan, Kazakhstan
Department of Biomedical Cell Technologies, Far Eastern Federal University, Vladivostok, Russian Federation

Special Centre for Molecular Medicine
Medicinal Chemistry Laboratory
Center for Translational Cancer Research Technische
Institute of Cytology of the Russian Academy of Sciences (RAS)
Department of General Surgery
Almazov National Medical Research Centre
National Center for Neurosurgery
Department of Biomedical Cell Technologies

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