Genetic Diversity of Pilin From Kazakh Isolates of Moraxella bovoculi
Kuibagarov M. Zhylkibayev A. Kamalova D. Ryskeldina A. Yerzhanova N. Ramankulov Y. Shevtsov A. Angelos J.A.
November 2022ResearchersLinks Ltd
Advances in Animal and Veterinary Sciences
2022#10Issue 112376 - 2383 pp.
Moraxella bovoculi (M. bovoculi) is commonly isolated from clinical cases of infectious bovine keratoconjunctivitis (IBK), however, the exact role of M. bovoculi in this disease remains unclear. Experimental Moraxella bovis (M. bovis) and M. bovoculi vaccines to prevent IBK based on recombinant pathogenic factors have been evaluated as alternatives to whole cell vaccines. Pilin is one candidate antigen for M. bovoculi, and is a key pathogenic factor necessary for attachment of M. bovis to the surface of the eye epithelium. To evaluate pilA diversity amongst Kazakh isolates of M. bovoculi, the pilA gene sequence was determined for 35 isolates of M. bovoculi. Ten genotypes (gNA1-gNA10) were identified which were 96.1% to 99.8% identical. It was found that as a result of the replacement of cytosine with thymine at the 97th position of the gNA8 genotype, a stop codon resulted which, if expressed, would have resulted in a truncated PilA. Phylogenetic analyses of the deduced amino acid sequences of PilA from isolates from Kazakhstan, in comparison with previously defined PilA groups in M. bovoculi, established that 68% of the Kazakh isolates belonged to group A, 8.6% to groups C and F. It was found that despite these isolates originating from geographically close regions, some of the studied isolates had unique amino acid sequences that were assigned to new M. bovoculi PilA groups designated K (2 isolates), L (2 isolates), and M (1 isolate).
Infectious bovine keratoconjunctivitis , Moraxella bovoculi , Pila , Pilin
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National Center for Biotechnology, 13/5,Kurgalzhynskoye road, Astana, 010000, Kazakhstan
Department of Medicine & Epidemiology, School of Veterinary Medicine, University of California, Davis, 95616, CA, United States
National Center for Biotechnology
Department of Medicine & Epidemiology
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