Transcriptomics and methylomics study on the effect of iodine-containing drug FS-1 on Escherichia coli ATCC BAA-196
Korotetskiy I.S. Jumagaziyeva A.B. Shilov S.V. Kuznetsova T.V. Myrzabayeva A.N. Iskakbayeva Z.A. Ilin A.I. Joubert M. Taukobong S. Reva O.N.
September 2021Future Medicine Ltd.
Future Microbiology
2021#16Issue 141063 - 1085 pp.
Aim: Promising results on application of iodine-containing nano-micelles, FS-1, against antibiotic-resistant Escherichia coli was demonstrated. Materials & methods: RNA sequencing for transcriptomics and the complete genome sequencing by SMRT PacBio were followed by genome assembly and methylomics. Results & conclusion: FS-1-treated E. coli showed an increased susceptibility to antibiotics ampicillin and gentamicin. Cultivation with FS-1 caused gene expression alterations toward anaerobic respiration, increased anabolism and inhibition of many nutrient uptake systems. Main targets of iodine-containing particles were cell membrane structures causing oxidative, osmotic and acidic stresses. Identification of methylated nucleotides showed an altered pattern in the FS-1-treated culture. Possible role of transcriptional and epigenetic modifications in the observed increase in susceptibility to gentamicin and ampicillin were discussed.
antibiotic resistance , complete genome sequence , epigenetics , Escherichia coli , HPLC , iodine , nano-micelle , transcriptomics
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Department of Virology, Scientific Center for Anti-Infectious Drugs, Almaty, Kazakhstan
Department of Microbiology, Scientific Center for Anti-Infectious Drugs, Almaty, Kazakhstan
Department of Chemistry, Scientific Center for Anti-Infectious Drugs, Almaty, Kazakhstan
Department of Biochemistry, Genetics and Microbiology, Centre for Bioinformatics and Computational Biology, University of Pretoria, Pretoria, South Africa
Department of Virology
Department of Microbiology
Department of Chemistry
Department of Biochemistry
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