Micropropagation of Cotoneaster melanocarpus Fisch. ex A.Blytt: an economically important ornamental plant

Kirillov V. Pathak A. Patel S.R. Daulenova M. Dyussembekova D. Stikhareva T. Rakhimzhanov A. Kakimzhanova A.
February 2023 Springer

In Vitro Cellular and Developmental Biology - Plant
2023 #59 Issue 1 147 - 153 pp.

Cotoneaster melanocarpus Fisch. ex A.Blytt (Rosaceae) is a deciduous shrub that is difficult to propagate through traditional methods. Thus, in the present study, in vitro regeneration utilizing nodal explants was attempted for the first time. Murashige and Skoog (MS) and Quoirin and Lepoivre (QL) medium supplemented with 0.2 or 0.5 mg L⁻¹ 6-benzyladenine (BA), 0.01 mg L⁻¹ indole-3-butyric acid (IBA), and 0.5 mg L⁻¹ gibberellic acid (GA₃) were utilized for shoot culture establishment. Optimum 8.83 ± 0.66 shoots with 0.87 ± 0.10 cm length and 4.27 ± 0.37 leaves per explant were recorded after 40 d in MS medium fortified with 0.2 mg L⁻¹ BA, 0.01 mg L⁻¹ IBA, and 0.5 mg L⁻¹ GA₃. These shoots were then rooted in ½MS medium augmented with 0.5 mg L⁻¹ IBA which formed 4.03 ± 0.29 roots per shoot (100% response) with 3.64 ± 0.16 cm length. The plantlets were then transferred to soil:vermiculite (3:1 w/w) in the light room for 35 d. Later on, they were successfully hardened and acclimatized in open ground with 80.9% survival rate after 3 mo.

Cotoneaster melanocarpus , Hardening and acclimatization , In vitro multiplication , Medicinal plant , Ornamental plant

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Department of Breeding, A.N. Bukeikhan Kazakh Research Institute of Forestry and Agroforestry, Shchuchinsk, Kazakhstan
Department of Botany, University of Rajasthan, Rajasthan, Jaipur, India
Department of Botany, Faculty of Science, The Maharaja Sayajirao University of Baroda, Gujarat, Vadodara, India
Laboratory of Plants Biotechnology and Breeding, National Center for Biotechnology, Astana, Kazakhstan

Department of Breeding
Department of Botany
Department of Botany
Laboratory of Plants Biotechnology and Breeding

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