Preliminary Laboratory Assessment of Agricultural Waste-Based Microbial Immobilization for Oil Degradation: A Screening Study

Khozhanepessova F. Serikbayeva A. Amankeshuly D. Koibakova S. Sagindykova E. Dadrasnia A. Myrzabekova A.
1 December 2025 Institute for Biodiversity and Ecology

Ecologica Montenegrina
2025 #85 Issue 2 Special Issue 141 - 149 pp.

Oil contamination in arid regions poses significant environmental challenges under high salinity and extreme temperatures. This study investigated bioremediation performance of free and carrier-immobilized bacterial consortia (Rhodococcus erythreus AT7 and Dietzia maris 22K) under varying temperature (+4°C, +32°C, +42°C) and salinity conditions (3%, 5%, 10% NaCl; 0.5%, 1% MgSO₄). Strains were immobilized on agricultural waste carriers (buckwheat and rice husks) using adsorption method. Rice husk-immobilized bacteria demonstrated superior oil degradation, achieving 78.4±0.89% efficiency under extreme stress (10% NaCl, +42°C) versus 37.2±0.95% for free cells. Optimal temperature (+32°C) enhanced degradation rates, with immobilized systems maintaining 75.0-83.0% efficiency under high salinity. Temperature elevation to +42°C severely impacted free cell viability (5.1 log CFU/g) while immobilized systems maintained higher densities (6.9 log CFU/g). Results demonstrate that agricultural waste-based immobilization significantly enhances microbial stress tolerance and bioremediation efficiency, offering practical solutions for petroleum contamination treatment under extreme environmental conditions. Field validation is required before practical application.

Agricultural Waste , Bacterial survival , Biocarrier , Hydrocarbon degradation , Microbial Immobilization , Thermal stress

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Department of Ecology and Geology, Faculty of Engineering, Yessenov University, 32 microdistrict, building 1, Aktau, Kazakhstan
BETA Tech Center, University of Vic-Central University of Catalonia, C.de La Laura 13, Vic, 08500, Spain
Al-Farabi Kazakh National University, Al-Farabi Avenue 71, Almaty, Kazakhstan

Department of Ecology and Geology
BETA Tech Center
Al-Farabi Kazakh National University

10 лет помогаем публиковать статьи Международный издатель

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