Ceratonia siliqua L. pod Effects on Viability Gene Expression of Endometrial Mesenchymal Stromal/Stem Cells Isolated from Women with Endometriosis-Associated Infertility
Khodabandeh Z. Jahromi B.N. Hashemi A. Hessami K. Jamhiri I. Zare S. Badr P. Iraji A. Poordast T. Baghban N. Khoradmehr A. Mussin N.M. Kaliyev A.A. Iztleuov Y.M. Shirazi R. Mahdipour M. Bakhshalizadeh S. Rahmanifar F. Jafari N. Tanideh N. Tamadon A.
October 2024Royan Institute (ACECR)
International Journal of Fertility and Sterility
2024#18Issue 4391 - 403 pp.
Background: This study aims to investigate the effects of carob (Ceratonia siliqua L.) pod extract (CPE) on the viability of human endometrial mesenchymal stromal/stem cells (EnMSCs) and its impact on mRNA and protein expressions of DNA methyltransferases (DNMT1, DNMT3A, and DNMT3B), histone deacetylase 1 (HDAC1), matrix metalloproteinase-2 (MMP2), and cyclooxygenase-2 (COX-2) in endometriotic patients. Materials and Methods: In this experimental study, EnMSCs were derived from endometrium of patients with ovarian endometrioma (OMA-EnMSCs group) and deep infiltrative endometriosis (DIE) samples of 10 endometriosisassociated infertility (EAI) women (E-EnMSCs group) and compared to EnMSCs derived from the endometrium of an endometriosis-free, normal woman as the control group (C-EnMSCs). The metabolic activity of the control and case groups were evaluated by treating them with different concentrations of CPE. Cell viability was analysed by MTT. Real-time reverse transcription-polymerase chain reaction (RT-PCR) and Western blot were used to evaluate the expression of specific genes at the mRNA and protein levels, respectively. Results: Treatment with 0.8 and 2 μg/mL of CPE downregulated COX-2 and HDAC1 in the E-EnMSC group compared to the C-EnMSCs group. Treatment with 0.8 μg/mL of CPE also decreased MMP2 and DNMT3B gene expressions. The COX-2 and DNMT3A genes were significantly upregulated after treatment with 2 μg/mL of CPE. Expressions of the COX-2, HDAC1, DNMT1, DNMT3A, and DNMT3B peptides decreased in the all three groups after treatment with 0.8 and 2 μg/mL of CPE. Gas chromatography-mass spectroscopy (GC-MS) analysis of CPE identified 14 bioactive compounds. Molecular docking showed the best position of each bioactive compound on the different target proteins that are involved in the process of apoptosis in EnMSCs. Conclusion: In vitro and in silico analyses of CPE bioactive compounds show that they may downregulate the cell inflammatory pathway involved in the pathophysiology of endometriosis.
Carob , Endometriosis , Gene Expression , Mesenchymal , Stem Cells
Text of the article Перейти на текст статьи
Stem Cell Technology Research Centre, Shiraz University of Medical Sciences, Shiraz, Iran
Infertility Research Centre, Shiraz University of Medical Sciences, Shiraz, Iran
Department of Obstetrics and Gynaecology, Shiraz School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran
Maternal-Foetal Medicine Research Centre, Shiraz University of Medical Sciences, Shiraz, Iran
Pharmaceutical Sciences Research Centre, Shiraz University of Medical Sciences, Shiraz, Iran
Phytopharmaceutical Technology and Traditional Medicine Incubator, Shiraz University of Medical Sciences, Shiraz, Iran
Medicinal and Natural Products Chemistry Research Centre, Shiraz University of Medical Sciences, Shiraz, Iran
Central Research Laboratory, Shiraz University of Medical Sciences, Shiraz, Iran
The Persian Gulf Marine Biotechnology Research Centre, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran
General Surgery, West Kazakhstan Marat Ospanov Medical University, Aktobe, Kazakhstan
Department of Oncology, West Kazakhstan Marat Ospanov Medical University, Aktobe, Kazakhstan
Department of Anatomy, School of Biomedical Sciences, Medicine and Health, UNSW Sydney, Sydney, Australia
Stem Cell Research Centre, Tabriz University of Medical Sciences, Tabriz, Iran
Department of Applied Cell Sciences, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran
Reproductive Development, Murdoch Children’s Research Institute, Melbourne, VIC, Australia
Department of Paediatrics, University of Melbourne, Melbourne, VIC, Australia
Department of Basic Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
PerciaVista R&D Co, Shiraz, Iran
Department of Pharmacology, Medical School, Shiraz University of Medical Sciences, Shiraz, Iran
Department of Natural Sciences, West Kazakhstan Marat Ospanov Medical University, Aktobe, Kazakhstan
Stem Cell Technology Research Centre
Infertility Research Centre
Department of Obstetrics and Gynaecology
Student Research Committee
Maternal-Foetal Medicine Research Centre
Pharmaceutical Sciences Research Centre
Phytopharmaceutical Technology and Traditional Medicine Incubator
Medicinal and Natural Products Chemistry Research Centre
Central Research Laboratory
The Persian Gulf Marine Biotechnology Research Centre
General Surgery
Department of Oncology
Department of Anatomy
Stem Cell Research Centre
Department of Applied Cell Sciences
Reproductive Development
Department of Paediatrics
Department of Basic Sciences
PerciaVista R&D Co
Department of Pharmacology
Department of Natural Sciences
10 лет помогаем публиковать статьи Международный издатель
Книга Публикация научной статьи Волощук 2026 Book Publication of a scientific article 2026