Synthesis and multifaceted characterization of bioactive Co-Y-Fe layered double hydroxide: bridging peroxidase-mimic function with antibacterial and anticancer efficacy in a unified strategy
Khataee S. Khataee A. Rashtbari S. Arefi-Oskoui S. Kudaibergenov N. Alikeyeva A. Dehghan G.
1 December 2025Elsevier Ltd
Applied Clay Science
2025#277
Increasing antibiotic-resistant infections and rapidly progressing cancers have led to a serious demand for more effective platforms to overcome the challenges related to conventional therapies. The catalytic reaction between nanozymes and hydrogen peroxide (H2O2) generates reactive oxygen species (ROSs), which effectively break down bacterial cell walls and induce apoptosis in cancer cells without causing significant off-target effects. Accordingly, nanozymes have emerged as promising nanostructures for biomedical and antibacterial applications. Herein, a novel cobalt(II)‑yttrium(III)‑iron(III) layered double hydroxide (Co-Y-Fe LDH) was synthesized and characterized in terms of structure, composition, and surface properties. After investigating the enzyme-mimic performance, the peroxidase-based antibacterial efficiency was extensively evaluated against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus), showing a minimum inhibitory concentration (MIC) of Co-Y-Fe LDH to be 38.7 ± 0.53 μg mL−1 and 33.1 ± 0.41 μg mL−1, respectively. Furthermore, the cytotoxic evaluations exhibited potent anticancer activity against cancer cells with a half-maximal inhibitory concentration (IC50) of 25.01 ± 1.2 μg mL−1. Meanwhile, the case-studied nanozyme exhibited insignificant toxicity towards the human normal cells. These results indicate the multifunctional capabilities of Co-Y-Fe LDH as an effective antibacterial and anticancer agent with a biocompatible nature for a wide range of applications.
Antibacterial , Anticancer , Cytotoxicity , Layered double hydroxide , Nanozymes , Peroxidase-mimicking
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Laboratory of Biochemistry and Molecular Biology, Department of Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, 51666−16471, Iran
Research Laboratory of Advanced Water and Wastewater Treatment Processes, Department of Applied Chemistry, Faculty of Chemistry, University of Tabriz, Tabriz, 51666−16471, Iran
Department of Chemical Engineering, Istanbul Technical University, Istanbul, 34469, Turkey
Center of Physical Chemical Methods of Research and Analysis, Department of Chemistry and Chemical Technology, Al-Farabi Kazakh National University, Al-Farabi 71, Almaty, 050038, Kazakhstan
Laboratory of Biochemistry and Molecular Biology
Research Laboratory of Advanced Water and Wastewater Treatment Processes
Department of Chemical Engineering
Center of Physical Chemical Methods of Research and Analysis
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