Retrotransposon-Based Genetic Diversity of Rhodiola rosea L. (Crassulaceae) from Kazakhstan Altai
Khapilina O. Turzhanova A. Zhumagul M. Tagimanova D. Raiser O. Kubentayev S. Shevtsov V. Hohn M.
January 2025Multidisciplinary Digital Publishing Institute (MDPI)
Diversity
2025#17Issue 1
The analysis of genetic diversity in natural populations of valuable medicinal plant species experiencing overexploitation is a key aspect of their natural conservation strategy. Mobile genetic elements and other interspersed repeats, which are major components of eukaryotic genomes, serve as effective tools for studying plant biodiversity and variability. The genetic diversity of four valuable medicinal plant Rhodiola rosea L. populations was investigated using the inter-repeat amplified PCR method with inter-priming binding sites (iPBSs) for genome profiling. At the interpopulation level, unique amplicons characteristic of specific R. rosea populations were identified. Molecular variance analysis revealed that the biodiversity of R. rosea populations in the Kazakh Altai region is 56% attributed to interpopulation differences and 44% to intrapopulation differences. It was shown that populations located in favorable environmental conditions have greater genetic diversity compared to those in extreme habitats. This study identified a high degree of polymorphism among R. rosea populations using the inter-repeat amplified PCR method. The genetic diversity of the populations ranged from 0.105 to 0.156, with an average heterozygosity of 0.134. The findings provide new insights into the population structure of R. rosea in the Kazakh Altai, enabling the identification of different genotypes, which will significantly complement traditional methods for conserving this valuable medicinal plant.
genetic diversity , iPBS profiling , medicinal plant , molecular marker , Rhodiola rosea
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National Center for Biotechnology, Qorghalzhyn Hwy 13, Astana, 010000, Kazakhstan
Department of Graduate School of Natural Sciences, Astana International University, Kabanbai Batyr 8, Astana, 010000, Kazakhstan
Astana Botanical Garden, Orynbor 16, Astana, 010000, Kazakhstan
Department of Botany, Institute of Agronomy, Hungarian University of Agriculture and Life Sciences, Budapest, -1118, Hungary
National Center for Biotechnology
Department of Graduate School of Natural Sciences
Astana Botanical Garden
Department of Botany
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