Exploring Genetic Diversity and Inter-/Intraspecific Polymorphism in Rheum sp. (Polygonaceae) Using the iPBS Retrotransposon Marker System
Khapilina O.N. Turzhanova A.S. Gemejieva N.G. Sumbembayev A.A. Arysbayeva R.B. Magzumova S. Kudrina N.O. Kulmanov T.E. Mamirova A. Terletskaya N.V.
September 2025Multidisciplinary Digital Publishing Institute (MDPI)
International Journal of Molecular Sciences
2025#26Issue 18
This study investigated interspecific and intraspecific polymorphism in Rheum (Polygonaceae) from Kazakhstan using the inter-primer binding site (iPBS) retrotransposon marker system. The results revealed considerable variation in the level and nature of genetic polymorphism both within and among Rheum species and ecopopulations across different regions of Kazakhstan. Rh. compactum and the ecopopulation Rh. tataricum from the Zhambyl Region (ZH) exhibited the lowest levels of polymorphism, supporting their designation as conservation priorities. Genetic differentiation analysis among species and ecopopulations identified clear distinctions, resulting in the formation of well-defined clusters with high bootstrap support. Minimal genetic distances were observed between the two ecopopulations of Rh. tataricum, along with a high degree of intraspecific genetic homogeneity in Rh. compactum and Rh. nanum. A distinct genetic divergence between Rh. compactum and the other taxa was detected, reinforcing its status as a separate species rather than a synonym of Rh. altaicum. The iPBS markers proved effective for investigating genetic variation in Rheum, offering valuable insights for future studies aimed at understanding the evolutionary history of the genus.
polymorphism , retrotransposon marker system , Rh. compactum , Rheum species
Text of the article Перейти на текст статьи
National Center for Biotechnology, Korghalzhyn 13, Astana, 010000, Kazakhstan
Institute of Botany and Phytointroduction, Timiryazev 36, Almaty, 050040, Kazakhstan
Altai Botanical Garden, Ermakov 1, Ridder, 071300, Kazakhstan
Faculty of Biology and Biotechnology, Al-Farabi Kazakh National University, Al-Farabi 71/19, Almaty, 050040, Kazakhstan
Institute of Genetic and Physiology, Al-Farabi 93, Almaty, 050040, Kazakhstan
National Center for Biotechnology
Institute of Botany and Phytointroduction
Altai Botanical Garden
Faculty of Biology and Biotechnology
Institute of Genetic and Physiology
10 лет помогаем публиковать статьи Международный издатель
Книга Публикация научной статьи Волощук 2026 Book Publication of a scientific article 2026