Optimized Food-Grade Semi-Synthetic Culture Medium and Freeze-Drying Approach with Antidiabetic Cryoprotectants for Akkermansia Muciniphila
Khamitkyzy Z. Kistaubayeva A. Savitskaya I. Shokatayeva D. Zhantlessova S.
September 2025Engineered Science Publisher
ES Food and Agroforestry
2025#21
Akkermansia Muciniphila is a mucin-degrading intestinal bacterium known for its beneficial effects, including antidiabetic and antiobesity effects. It typically grows on media supplemented with animal-derived mucin. However, the use of such substrates in cultivation is unsuitable for food applications due to ethical and dietary concerns. To address this, we developed a plant-based, semi-synthetic medium using soy and pea peptones along with L-threonine as nitrogen sources, and N-acetylglucosamine with glucose as carbon substrates. The strain reached high cell density (4.85×10⁸ CFU/mL) on this medium, exceeding yields typically observed in mucin-based media. The medium composition was optimized using response surface methodology, resulting in the following formulation: 1.5 g/L threonine, 10 g/L soy peptone, 15 g/L pea peptone, 5.0 g/L glucose, 7.0 g/L GlcNAc, pH 7.0. The obtained biomass was lyophilized with cryoprotectants of camel milk and D-allulose. Cell viability in the final freeze-dried powder was 84.2%, with minimal membrane damage confirmed by flow cytometry. Our results support the use of this animal-free system for the development of functional foods containing A. muciniphila.
Akkermansia Muciniphila , Antidiabetic , Cryoprotectants , Food-grade , Semi-synthetic medium
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Department of Biotechnology, Al-Farabi Kazakh National University, 71 Al-Farabi Avenue, Almaty, 050040, Kazakhstan
Department of Biotechnology
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