Exploring the Chemical Composition and Antimicrobial Activity of Extracts from the Roots and Aboveground Parts of Limonium gmelini
Kassymova D. Cairone F. Ambroselli D. Lanzetta R. Casciaro B. Zhussupova A. Quaglio D. Casillo A. Zhusupova G.E. Corsaro M.M. Botta B. Cammarone S. Mangoni M.L. Ingallina C. Ghirga F.
July 2025Multidisciplinary Digital Publishing Institute (MDPI)
Molecules
2025#30Issue 14
Limonium gmelini (Willd.) Kuntze, a plant widely used in traditional medicine, has garnered increasing attention for its diverse pharmacological activities, including anti-inflammatory, hepatoprotective, antioxidant, and antimicrobial effects. This study aimed to explore the chemical composition and biological activities of polysaccharides and polyphenolic compounds extracted from both the roots and aboveground parts of Limonium gmelini. Several methods of extraction, including ultrasound-assisted extraction (UAE), conventional maceration (CM), and supercritical fluid extraction (SFE), were employed to obtain bioactive fractions. Chemical profiling, primarily represented by monosaccharides and polyphenolic compounds, was characterized and analyzed using proton nuclear magnetic resonance spectroscopy (1H-NMR) and gas chromatography-mass spectrometry (GC-MS) techniques. While polyphenol-rich fractions exhibited significant antibacterial activity, particularly against Staphylococcus epidermidis, polysaccharide-rich aqueous fractions showed minimal antibacterial activity. Among the methods, CM and UAE yielded higher polyphenol content, whereas SFE provided more selective extractions. Notably, methanolic SPE fractions derived from the roots were especially enriched in active polyphenols such as gallic acid, myricetin, and naringenin, and they exhibited the highest antibacterial activity against Staphylococcus epidermidis. In contrast, extracts from the aboveground parts showed more moderate activity and a partially different chemical profile. These findings underscore the importance of plant part selection and support the targeted use of root-derived polyphenol-enriched fractions from L. gmelini as promising candidates for the development of natural antibacterial agents. Further investigation is needed to isolate and validate the most active constituents for potential therapeutic applications.
antibacterial activity , Limonium gmelini , monosaccharide composition , monosaccharide linkages , polyphenols , polysaccharides
Text of the article Перейти на текст статьи
Department of Chemistry and Technology of Organic Substances, Natural Compounds and Polymers, Faculty of Chemistry and Chemical Technology, NPJSC Al-Farabi Kazakh National University, Al-Farabi Ave. 71, Almaty, 050040, Kazakhstan
Department of Chemistry and Technology of Drugs, Sapienza University of Rome, Piazzale Aldo Moro 5, Rome, 00185, Italy
Department of Chemical Sciences, Complesso Universitario di Monte S. Angelo, University of Napoli Federico II, Via Cintia 4, Napoli, 80126, Italy
Laboratory Affiliated to Istituto Pasteur Italia-Fondazione Cenci Bolognetti, Department of Biochemical Sciences, Sapienza University of Rome, Rome, 00185, Italy
Department of Molecular Biology and Genetics, Faculty of Biology and Biotechnology, NPJSC Al-Farabi Kazakh National University, Al-Farabi Ave. 71, Almaty, 050040, Kazakhstan
Department of Chemistry and Technology of Organic Substances
Department of Chemistry and Technology of Drugs
Department of Chemical Sciences
Laboratory Affiliated to Istituto Pasteur Italia-Fondazione Cenci Bolognetti
Department of Molecular Biology and Genetics
10 лет помогаем публиковать статьи Международный издатель
Книга Публикация научной статьи Волощук 2026 Book Publication of a scientific article 2026