AtDREB2A gene expression under control of the inducible promoter and virus 5’-untranslated regions improves tolerance to salinity in Nicotiana tabacum

Karpova O. Alexandrova A. Nargilova R. Ramazanova M. Kryldakov R. Iskakov B.
2021 North Atlantic University Union NAUN

International Journal of Biology and Biomedical Engineering
2021 #15 260 - 274 pp.

Transcriptional factor DEHYDRATION-RESPONSIVE ELEMENT BINDING PROTEIN 2A (DREB2A) induces the expression of many genes in dehydration, heat shock, and salinity in Arabidopsis. Deletion of sequence coding the 30 amino acid central region transforms full length (FL) protein DREB2A FL into a more stable and constitutively active form known as DREB2A CA. Here, using agrobacteria, a leaf disc transformation of Nicotiana tabacum v. Samsun NN was carried out by transgenes AtDREB2A-FL and AtDREB2A-CA coding the proteins with His-tag on the С-end. The effects of combinations of constitutive 35S CaMV promoter or inducible rd29A promoter with different viral or artificial 5’-untranslated regions (UTR), 5’TMV, 5’PVY, 5’AMV or 5’ARC1, known as translational enhancers were evaluated on the both transgenes’ expression. Using an antibody to His-tag, recombinant protein synthesis was detected in transgenic plants in normal and heat shock conditions. After comparative analysis, it was shown that the properties of different 5’-UTRs vary greatly and depended on separate conjunction of promoter and transgene. The integration of AtDREB2A CA under control of the rd29A promoter and 5’TMV or 5’AMV in genome effectively improved tolerance of tobacco transgenic plants to 400 mM NaCl and to drought.

5’-untranslated region , Abiotic stress , Transcriptional factor , Transgene , Translation enhancer

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M.А. Aitkhozhin Institute of Molecular Biology and Biochemistry, Dosmukhamedov str., 80, Almaty, 050012, Kazakhstan
Al-Farabi Kazakh National University, Timiryazev str., 71, Almaty, 050040, Kazakhstan

M.А. Aitkhozhin Institute of Molecular Biology and Biochemistry
Al-Farabi Kazakh National University

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