Distribution of Wheat-Infecting Viruses and Genetic Variability of Wheat Streak Mosaic Virus and Barley Stripe Mosaic Virus in Kazakhstan
Kapytina A. Kolchenko M. Kerimbek N. Pozharskiy A.S. Nizamdinova G. Taskuzhina A. Adilbayeva K. Khusnitdinova M. Amidullayeva M. Moisseyev R. Kachiyeva Z. Gritsenko D.
January 2024Multidisciplinary Digital Publishing Institute (MDPI)
Viruses
2024#16Issue 1
Wheat is an essential cereal crop for the economy and food safety of Kazakhstan. In the present work, a screening of wheat and barley from different regions of Kazakhstan was conducted using newly developed specific primers for reverse transcription PCR and loop-mediated isothermal amplification (LAMP) assays. In total, 82 and 19 of 256 samples of wheat and barley tested positive for wheat streak mosaic virus (WSMV) and barley stripe mosaic virus (BSMV), respectively. A phylogenetic analysis using two independent methods revealed that most of the analyzed isolates had a European origin. Molecular data on the distribution and diversity of cereal viruses in Kazakhstan were obtained for the first time and will help lay a foundation for the implementation of genetics and genomics in wheat phyto-epidemiology in the country.
BSMV , coat protein , WSMV , γB protein
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Laboratory of Molecular Biology, Institute of Plant Biology and Biotechnology, Almaty, 050040, Kazakhstan
Department of Molecular Biology and Genetics, Al Farabi Kazakh National University, Almaty, 050040, Kazakhstan
Research Institute of Applied and Fundamental Medicine, Kazakh National Medical University, Almaty, 050000, Kazakhstan
Laboratory of Molecular Biology
Department of Molecular Biology and Genetics
Research Institute of Applied and Fundamental Medicine
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