Recent advances and challenges in plant viral diagnostics
Kanapiya A. Amanbayeva U. Tulegenova Z. Abash A. Zhangazin S. Dyussembayev K. Mukiyanova G.
2024Frontiers Media SA
Frontiers in Plant Science
2024#15
Accurate and timely diagnosis of plant viral infections plays a key role in effective disease control and maintaining agricultural productivity. Recent advances in the diagnosis of plant viruses have significantly expanded our ability to detect and monitor viral pathogens in agricultural crops. This review discusses the latest advances in diagnostic technologies, including both traditional methods and the latest innovations. Conventional methods such as enzyme-linked immunosorbent assay and DNA amplification-based assays remain widely used due to their reliability and accuracy. However, diagnostics such as next-generation sequencing and CRISPR-based detection offer faster, more sensitive and specific virus detection. The review highlights the main advantages and limitations of detection systems used in plant viral diagnostics including conventional methods, biosensor technologies and advanced sequence-based techniques. In addition, it also discusses the effectiveness of commercially available diagnostic tools and challenges facing modern diagnostic techniques as well as future directions for improving informed disease management strategies. Understanding the main features of available diagnostic methodologies would enable stakeholders to choose optimal management strategies against viral threats and ensure global food security. Copyright
biosensor , CRISPR-Cas , disease diagnostics , plant virus , sequencing technologies
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Department of Biotechnology and Microbiology, L.N. Gumilyov, Eurasian National University, Astana, Kazakhstan
Laboratory of Biodiversity and Genetic Resources, National Center for Biotechnology, Astana, Kazakhstan
Scientific Center Agrotechnopark, Shakarim University, Semey, Kazakhstan
Department of Biotechnology and Microbiology
Laboratory of Biodiversity and Genetic Resources
Scientific Center Agrotechnopark
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