Effect of the photosynthesis inhibitors on hydrogen production by non-heterocyst cyanobacterial strains
Kamshybayeva G.K. Kossalbayev B.D. Sadvakasova A.K. Bauenova M.O. Zayadan B.K. Krapivina A.A. Sainova G.A. Alharby H.F. Allakhverdiev S.I.
2 January 2024Elsevier Ltd
International Journal of Hydrogen Energy
2024#52167 - 182 pp.
The energy of cyanobacterial hydrogen (H2) produced via bio-photolysis is being investigated as a potential solution to early-century environmental challenges. The main limiting factors of cyanobacterial H2 photoproduction are the availability of electrons for [NiFe]-hydrogenase (H2ase) and the suppression of bidirectional H2ase activity induced by O2 acquired from water molecules splitting in photosystem II. The current study investigated how photosynthetic inhibitors (PIs) affected H2 production in non-N2-fixing cyanobacteria. Study findings revealed a rather high H2 yield in Synechocystis sp. PSU 1262, as well as a beneficial (14.2-fold) influence of 500 μmol KCN on the H2 production by the aforesaid strain. A 12/6-h light/dark cycle increased H2 production by 80.3% in cells supplemented with 500 μmol KCN. Under the optimised conditions, the photobiological H2 production of Synechocystis sp. PSU 1262 increased from 49.6 to 1552 nmol H2 mg−1 Chl a h−1. PIs suppressed chlorophyll a concentration under illumination, lowering the O2 levels, which enhanced bidirectional H2ase activity in Synechocystis sp. PSU 1262 cells. Applying varied light modes, preceded by the incorporation of PIs at optimal concentrations in H2 production by research cyanobacterial strains, improved the H2 yield and contributed significantly to the research originality.
Biohydrogen , Cyanobacteria , Hydrogenase , Isolation , Photosynthetic inhibitors
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Faculty of Biology and Biotechnology, Al-Farabi Kazakh National University, Almaty, 050038, Kazakhstan
Department of Chemical and Biochemical Engineering, Institute of Geology and Oil-Gas Business Institute Named After K. Turyssov, Satbayev University, Almaty, 050043, Kazakhstan
K.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya Street 35, Moscow, 127276, Russian Federation
Ecology Research Institute, Khoja Akhmet Yassawi International Kazakh-Turkish University, Turkistan, 161200, Kazakhstan
Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah, 21589, Saudi Arabia
Institute of Basic Biological Problems, FRC PSCBR Russian Academy of Sciences, Pushchino, 142290, Russian Federation
Faculty of Engineering and Natural Sciences, Bahcesehir University, Istanbul, Turkey
Faculty of Biology and Biotechnology
Department of Chemical and Biochemical Engineering
K.A. Timiryazev Institute of Plant Physiology
Ecology Research Institute
Department of Biological Sciences
Institute of Basic Biological Problems
Faculty of Engineering and Natural Sciences
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