Electroelution Into a Salt Trap: Reviving an Old-School Approach to DNA Purification
Kalendar R. Ivanov K.I. Samuilova O.V. Burster T. Zamyatnin A.A., Jr.
9 March 2026John Wiley and Sons Inc
Small Methods
2026#10Issue 5
Recent advances in bioinstrumentation, such as the development of long-read sequencing, have reignited interest in methods for extracting long, intact nucleic acids from complex samples. One traditional method for this purpose is gel electrophoresis followed by electroelution from gel slices into a salt cushion. However, this method has become largely overlooked because its standard implementation is laborious, time-consuming, and incompatible with high-throughput workflows. In our recent work, we revisited this experimental approach and developed a simple, fast, and efficient method for purifying intact nucleic acids of varying lengths from complex samples. The method is available in both horizontal and vertical electrophoresis configurations, has the potential for automation and scalability, and is suitable for purifying high molecular weight (HMW) DNA for long-read sequencing. In this paper, we discuss the origins of the method, the stages of its development, and its advantages. The successful implementation of the method demonstrates how looking at a traditional technique from a new perspective can help meet the demands of next-generation technologies such as long-read sequencing. Our results highlight the importance of rethinking the applications of well-established yet underutilized methodological approaches in rapidly evolving fields such as biotechnology and bioinstrumentation.
agarose gel electrophoresis , DNA and RNA purification , electroelution , high molecular weight DNA , long-read DNA sequencing , nucleic acid , salt trap
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National Laboratory Astana, Nazarbayev University, Astana, Kazakhstan
Institute of Biotechnology, Helsinki Institute of Life Science (HiLIFE), University of Helsinki, Helsinki, Finland
Department of Microbiology, University of Helsinki, Helsinki, Finland
Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, Moscow, Russian Federation
Department of Biochemistry, Sechenov First Moscow State Medical University, Moscow, Russian Federation
Department of Biology, School of Sciences and Humanities, Nazarbayev University, Astana, Kazakhstan
Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russian Federation
National Laboratory Astana
Institute of Biotechnology
Department of Microbiology
Faculty of Bioengineering and Bioinformatics
Department of Biochemistry
Department of Biology
Belozersky Institute of Physico-Chemical Biology
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