Circulating adiponectin levels, expression of adiponectin receptors, and methylation of adiponectin gene promoter in relation to Alzheimer’s disease
Kaiyrlykyzy A. Umbayev B. Masoud A.-R. Baibulatova A. Tsoy A. Olzhayev F. Alzhanova D. Zholdasbekova G. Davletov K. Akilzhanova A. Askarova S.
December 2022BioMed Central Ltd
BMC Medical Genomics
2022#15Issue 1
Background: The role of adiponectin (ADIPOQ) in Alzheimer’s disease (AD) has been documented, however, demonstrating controversial results. In this study, we investigated blood serum ADIPOQ levels, methylation of the adiponectin gene promoter, and adiponectin receptors (AdipoR1 and AdipoR2) expression in blood samples isolated from AD patients and healthy controls. Methods: We performed a case–control study including 248 subjects (98 AD patients and 150 healthy controls); ADIPOQ serum levels, AdipoR1, and AdipoR2 levels in PBMC were measured by ELISA Kits, and ADIPOQ gene methylation was analyzed using methyl-specific PCR. Results: Serum adiponectin levels were threefold higher in the AD group compared to the controls. We have also found a positive correlation between adiponectin and MMSE scores and high-density lipoprotein cholesterol (HDL-C) in AD patients. A significant difference in the proportion of methylation of the CpG sites at − 74 nt of the ADIPOQ gene promoter was detected in AD cases, and the levels of adiponectin in blood serum were significantly higher in methylated samples in the AD group compared to controls. The amount of AdipoR1 was significantly higher among AD subjects, while the expression of AdipoR2 did not vary between AD patients and controls. Conclusion: These findings may contribute to a deeper understanding of the etiological factors leading to the development of dementia and may serve as a basis for the development of predictive biomarkers of AD.
Adiponectin , AdipoR1 , AdipoR2 , Alzheimer’s disease , DNA methylation
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Center for Life Sciences, National Laboratory Astana, Nazarbayev University, 53 Kabanbay Batyr Avenue, Astana, Kazakhstan
Faculty of Medicine and Public Health, al-Farabi Kazakh National University, Almaty, Kazakhstan
Louisiana Tech University, Ruston, LA, United States
Department of Neurology and Psychiatry, Astana Medical University, Astana, Kazakhstan
Open Clinic, Astana, Kazakhstan
Karaganda State Medical University, Karaganda, Kazakhstan
Asfendiyarov Kazakh National Medical University, Almaty, Kazakhstan
Center for Life Sciences
Faculty of Medicine and Public Health
Louisiana Tech University
Department of Neurology and Psychiatry
Open Clinic
Karaganda State Medical University
Asfendiyarov Kazakh National Medical University
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