Dynamic Translational Landscape Revealed by Genome-Wide Ribosome Profiling under Drought and Heat Stress in Potato
Jian H. Wen S. Liu R. Zhang W. Li Z. Chen W. Zhou Y. Khassanov V. Mahmoud A.M.A. Wang J. Lyu D.
June 2023MDPI
Plants
2023#12Issue 12
The yield and quality of potatoes, an important staple crop, are seriously threatened by high temperature and drought stress. In order to deal with this adverse environment, plants have evolved a series of response mechanisms. However, the molecular mechanism of potato’s response to environmental changes at the translational level is still unclear. In this study, we performed transcriptome- and ribosome-profiling assays with potato seedlings growing under normal, drought, and high-temperature conditions to reveal the dynamic translational landscapes for the first time. The translational efficiency was significantly affected by drought and heat stress in potato. A relatively high correlation (0.88 and 0.82 for drought and heat stress, respectively) of the fold changes of gene expression was observed between the transcriptional level and translational level globally based on the ribosome-profiling and RNA-seq data. However, only 41.58% and 27.69% of the different expressed genes were shared by transcription and translation in drought and heat stress, respectively, suggesting that the transcription or translation process can be changed independently. In total, the translational efficiency of 151 (83 and 68 for drought and heat, respectively) genes was significantly changed. In addition, sequence features, including GC content, sequence length, and normalized minimal free energy, significantly affected the translational efficiencies of genes. In addition, 28,490 upstream open reading frames (uORFs) were detected on 6463 genes, with an average of 4.4 uORFs per gene and a median length of 100 bp. These uORFs significantly affected the translational efficiency of downstream major open reading frames (mORFs). These results provide new information and directions for analyzing the molecular regulatory network of potato seedlings in response to drought and heat stress.
drought stress , heat stress , potato , ribosome profiling , upstream open reading frames
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College of Agronomy and Biotechnology, Southwest University, Chongqing, 400715, China
State Cultivation Base of Crop Stress Biology for Southern Mountainous Land of Southwest University, Chongqing, 400715, China
Chongqing Key Laboratory of Biology and Genetic Breeding for Tuber and Root Crops, Chongqing, 400715, China
Department of Plant Protection and Quarantine, Faculty of Agronomy, S. Seifullin Kazakh Agrotechnical University, Zhenis Avenue, Astana, 010011, Kazakhstan
Department of Vegetable Crops, Faculty of Agriculture, Cairo University, Giza, 12613, Egypt
College of Agronomy and Biotechnology
State Cultivation Base of Crop Stress Biology for Southern Mountainous Land of Southwest University
Chongqing Key Laboratory of Biology and Genetic Breeding for Tuber and Root Crops
Department of Plant Protection and Quarantine
Department of Vegetable Crops
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