Proliferating and migrating effects of regenerating sea anemone Aulactinia stella cells-derived exosomes on human skin fibroblasts
Jafari N. Afshar A. Zare A. Salehpour A. Hashemi A. Zendehboudi F. Farrar Z. Mahdipour M. Khoradmehr A. Jahanfar F. Mussin N.M. Kaliyev A.A. Kameli A. Azari H. Nabipour I. Zhilisbayeva K.R. Tamadon A.
2025Taylor and Francis Ltd.
Natural Product Research
2025#39Issue 174916 - 4923 pp.
Regenerative effects of sea anemone-derived exosomes on human foreskin fibroblasts (HFFs) were investigated. Water-based extracts from regenerating Aulactinia stella tissue were collected at various time points, and exosomes were extracted after inducing wounds. Both the extract and exosomes were tested on HFF for proliferation and in vitro wound healing. In silico analysis explored protein–protein docking between regenerative exosome proteins and HFF receptors. The MTT (3-(4,5-dimethylthiazol-2yl)-2,5 diphenyltetrazolium bromide proliferation assay and in vitro wound healing test of aquatic extract showed proliferative effects on HFF cell lines, with the 60 μg/mL concentration significantly enhancing cell migration. Exosomes were characterised. Exosomes showed a significantly positive effect on cell proliferation and migration at the 50 µg/mL concentration 48 h post-wound induction. In silico analysis revealed potential binding affinities between exosome proteins and HFF receptors. In conclusion, optimised concentrations of A. stella-derived exosomes exhibited positive effects on HFF regeneration and migration, suggesting their potential in accelerating wound healing.
exosome , migration , regeneration , Sea anemone , wound healing
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PerciaVista R&D Co, Shiraz, Iran
Department of Research and Development, Student Research Committee, Bushehr University of Medical Sciences, Bushehr, Iran
Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Department of Applied Cell Sciences, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran
The Persian Gulf Marine Biotechnology Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran
Department of Surgery and Urology No. 2, West Kazakhstan Marat Ospanov Medical University, Aktobe, Kazakhstan
Department of Scientific Work, West Kazakhstan Marat Ospanov Medical University, Aktobe, Kazakhstan
Department of Natural Sciences, West Kazakhstan Marat Ospanov Medical University, Aktobe, Kazakhstan
PerciaVista R&D Co
Department of Research and Development
Stem Cell Research Center
Department of Applied Cell Sciences
The Persian Gulf Marine Biotechnology Research Center
Department of Surgery and Urology No. 2
Department of Scientific Work
Department of Natural Sciences
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