Assessment of Biological Properties of Recombinant Lumpy Skin Disease Viruses with Deletions of Immunomodulatory Genes
Issabek A. Bopi A. Kozhabergenov N. Khudaibergenova B. Sultankulova K. Chervyakova O.
October 2025Multidisciplinary Digital Publishing Institute (MDPI)
Viruses
2025#17Issue 10
Rational design of capripoxvirus-based vaccine vectors can be achieved by knockout of immunomodulatory genes. In this study, the effect of knockout of the immunomodulatory genes LSDV005, LSDV008 and LSDV066 on the replication of Lumpy skin disease virus in cell cultures and the immune response to an integrated foreign antigen were assessed. The knockout of genes was performed by homologous recombination under conditions of temporary dominant selection. It was found that single knockout of the LSDV005 gene and the LSDV008 gene did not affect the replicative activity of recombinant viruses in vitro (Atyrau-5 and Atyrau-B). Both single knockout of the LSDV066 gene and in combination with knockout of LSDV005 or LSDV008 led to a decrease in the replicative activity of recombinant LSDVs. The recombinant Atyrau-5J(IL18) with LSDV005 gene knockout induced production of antibodies to the integrated antigen in mice. Prime-boost vaccination with all studied recombinants increased the level of interferon-γ. In addition, during immunization with the recombinant Atyrau-5J(IL18) secretion of interleukin-2 was significantly increased. The study of the functions of immunomodulatory genes and their effect on the expression of inserted sequences of foreign antigens is promising for the creation of highly effective polyvalent vector vaccines for animals.
immunomodulatory gene , lumpy skin disease virus , vaccine vector
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Research Institute for Biological Safety Problems (RIBSP), National Holding QazBioPharm, Gvardeiskiy uts, 080409, Kazakhstan
National Academy of Sciences of the Kyrgyz Republic, Bishkek, 720010, Kyrgyzstan
Research Institute for Biological Safety Problems (RIBSP)
National Academy of Sciences of the Kyrgyz Republic
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