Biosynthesized silver nanoparticles and miR34a mimics mediated activation of death receptor in MCF-7 human breast cancer cell lines
Iqbal M.J. Rashid U. Javed Z. Hamid Z. Imran K. Kabeer A. Raza S. Almarhoon Z.M. Reiner Ž. Bagiu I.-C. Bagiu R.V. Sarac I. Sharifi-Rad J. Ydyrys A. Daştan S.D. Butnariu M. Cho W.C.
December 2022BioMed Central Ltd
Cancer Nanotechnology
2022#13Issue 1
Nano-biotechnology-based clinical applications to cure health-related issues have gained huge attention among the scientific community and hold great promise to limit cancer metastasis. In this study, green-derived silver nanoparticles were synthesized by using leaf extract of Litchi chinensis. Characterization of biosynthesized silver nanoparticles was performed by using UV–Vis spectroscopy, FTIR, XRD, EDS, and SEM analysis. The clinical application of green-drive nanoparticles was investigated by using MCF-7 cancer cell lines. MCF-7 breast cancer cell lines were analyzed against three different treatments. (i) Silver nanoparticles (AgNPs), (ii) miR34a mimics and (iii) Co-delivery of AgNPs and miR34a mimics. Cell viability was determined by MTT assay and, extraction of mRNA and cDNA synthesis were performed after successful cellular transfection. qRT-PCR was done for expression analysis of DR4 and DR5 upon exogenous delivery of all 3 treatments. Results indicate that L. chinensis leaves have a significant amount of phenolic and flavonoid contents and also possess massive antioxidant activity. The diameter of nanoparticles was observed in the range of 41–55 nm. It was concluded that green-derived silver nanoparticles can be a potential contributing agent for cancer prevention and are reported to upregulate the expression of DR4 and DR5 by 0.8-folds and 3.7-folds, respectively.
Breast cancer , DR4 , DR5 , MCF-7 cell lines , Silver nanoparticles synthesis
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Department of Biochemistry and Biotechnology, University of Gujrat, Gujrat, Pakistan
Department of Biotechnology, University of Sialkot, Sialkot, Pakistan
Office of Research Innovation and Commercialization, Lahore Garrison University, Lahore, Pakistan
Department of Chemistry, College of Science, King Saud University, P. O. Box 2455, Riyadh, 11451, Saudi Arabia
University Hospital Centre Zagreb, Zagreb, Croatia
Department of Microbiology, Victor Babes University of Medicine and Pharmacy of Timisoara, Timisoara, Romania
Multidisciplinary Research Center on Antimicrobial Resistance, Timisoara, Romania
Preventive Medicine Study Center, Victor Babes University of Medicine and Pharmacy of Timisoara Department of Microbiology, Timisoara, Romania
University of Life Sciences “King Mihai I” from Timisoara, 300645, Calea Aradului 119, Timis, Romania
Facultad de Medicina, Universidad del Azuay, Cuenca, Ecuador
Biomedical Research Centre, Al-Farabi Kazakh National University, Al-Farabi Av. 71, Almaty, 050040, Kazakhstan
Department of Biology, Faculty of Science, Sivas Cumhuriyet University, Sivas, 58140, Turkey
Beekeeping Development Application and Research Center, Sivas Cumhuriyet University, Sivas, 58140, Turkey
Department of Clinical Oncology, Queen Elizabeth Hospital, Kowloon, Hong Kong
Department of Biochemistry and Biotechnology
Department of Biotechnology
Office of Research Innovation and Commercialization
Department of Chemistry
University Hospital Centre Zagreb
Department of Microbiology
Multidisciplinary Research Center on Antimicrobial Resistance
Preventive Medicine Study Center
University of Life Sciences “King Mihai I” from Timisoara
Facultad de Medicina
Biomedical Research Centre
Department of Biology
Beekeeping Development Application and Research Center
Department of Clinical Oncology
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