Prevalence and genotypes distribution of virus hepatitis B and hepatitis delta virus in chronic liver diseases in Kazakhstan
Ilyassova B.S. Abzhaparova B. Smailova D.S. Bolatov A. Baymakhanov B. Beloussov V. Solomadin M. Shamsivaliyeva K. Alpysbayava G. Issakova G. Granica J. Mukushkina D. Sagatov I.Y. Kaniyev S.
December 2023BioMed Central Ltd
BMC Infectious Diseases
2023#23Issue 1
Background: The geographical distribution of hepatitis B virus (HBV) and hepatitis D virus (HDV) genotypes is uneven and has its own clinical and organizational implications for health systems. Despite the introduction of vaccination and successful antiviral therapy the prevalence of chronic hepatitis B (with or without delta agent) increased over the past 5 years. This study aimed for the first time to investigate the molecular epidemiology of HBV and HDV in Kazakhstan. Methods: Total 834 chronic hepatitis B (with or without delta agent) patients were included to the study from November 2017 to June 2019. The material was collected from the regional hepatological сenters from 13 cities of Kazakhstan. Genotyping of HBV/HDV isolates was carried out using phylogenetic analysis of null-binary sequences of Kazakhstani isolates, in comparison with the reference sequences. Nucleotide sequence alignment was performed using the ClustalW algorithm, the “neighbor-joining” method was used for the construction of phylogenetic trees and subsequent analysis. Results: Overall 341 samples were PCR-positive and genotyped for HBV. Comparison and phylogenetic analysis of nucleotide sequences of HBV isolates showed that they were represented by genotypes HBV-D (95.9%), HBV-A (3.5%) and HBV-C (0.6%). At the same time, the identity of the nucleotide sequences of Kazakhstani isolates were: HBV-D (95–100%); HBV-A (97.2–100%) and HBV-C (99%). 256 samples were PCR positive and genotyped for HDV, all of them belonged to genotype 1. Conclusion: This study describes for the first time the molecular epidemiology of HBV and HDV in Kazakhstan. The data obtained expand the knowledge of the global epidemiology of viruses; have potential implications for public health policy and for further clinical research on chronic hepatitis in Kazakhstan. Trial registration: ClinicalTrials.gov NCT05095181 (registered on 27/10/2021).
delta agent , Genotype , Hepatitis B virus , Hepatitis D virus , PCR sequencing , Phylogenetic analysis
Text of the article Перейти на текст статьи
JSC “National Scientific center of Surgery named after A.N.Syzganov”, Zheltoksan str. 62, Almaty, 050004, Kazakhstan
Asfendiyarov Kazakh National Medical University, Almaty, Kazakhstan
Astana Medical University, Astana, Kazakhstan
Molecular Genetics Laboratory “TreeGene”, Almaty, Kazakhstan
Karaganda Medical University, Karaganda, Kazakhstan
JSC “National Scientific center of Surgery named after A.N.Syzganov”
Asfendiyarov Kazakh National Medical University
Astana Medical University
Molecular Genetics Laboratory “TreeGene”
Karaganda Medical University
10 лет помогаем публиковать статьи Международный издатель
Книга Публикация научной статьи Волощук 2026 Book Publication of a scientific article 2026