Obtaining Edible Pullulan-based Films with Antimicrobial Properties
Ignatova L.V. Urazova Z.K. Brazhnikova Y.V. Vedyashkina N.V.
2024Institute of Combustion Problems, al-Farabi Kazakh State National University
Eurasian Chemico-Technological Journal
2024#25Issue 4241 - 247 pp.
A nutrient medium was selected for the efficient production of exopolysaccharide (EPS) by A. pullulans C7 strain. The production of pullulan polysaccharide was evaluated on nutrient media with traditional carbon sources and cheap substrates that were plant wastes. For maximum EPS accumulation, we proposed an optimized Czapek-Dox medium with glucose as a carbon source, sodium nitrate as a nitrogen source, and C/N=232:1 ratio (EPS yield 12.79±0.64 g/l). Medium with grape pomace 5% (EPS yield was 15.08±0.34 g/l) and medium with topinambour tuber hydrolysate 5% (EPS yield was 14.44±0.21 g/l) was proposed as a cheap substrate. Edible films with antimicrobial activity were obtained on the basis of the isolated polysaccharide. The antibacterial activity of films against Escherichia coli 603 and Staphylococcus aureus ST228 was shown when essential oils of rosemary (zones of growth inhibition from 8.41±0.71 to 9.98±0.32 mm) and oregano (zones of growth inhibition from 8.09±0.51 to 9.54±0.24 mm) were added to pullulan. The addition of xanthan gum and glycerol to the films increased their strength and elasticity. The infrared spectrum of the pullulan film showed absorption bands characteristic of polysaccharide structures.
Antibacterial activity , Edible film , Medium optimisation , Pullulan
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Al-Farabi Kazakh National University, 71, Al-Farabi Ave, Almaty, Kazakhstan
Scientific Research Institute of Biology and Biotechnology Problems, 71, Al-Farabi Ave, Almaty, Kazakhstan
Al-Farabi Kazakh National University
Scientific Research Institute of Biology and Biotechnology Problems
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