PDGFD maintains ovine tail ADSCs in a proliferative state by suppressing CXCL8 and activating PI3K/MAPK signaling

Han J. Liang Q. Zhang J. Wang X. Kulatayev B.T. Rouzi M. Wan P. Yang M.
2026 Frontiers Media SA

Frontiers in Veterinary Science
2026 #13

Introduction: The fat tail in sheep is a distinctive adaptive trait, yet the cellular mechanisms controlling its development remain poorly understood. Although PDGFD is a high-priority candidate gene from selective sweep analyses, its precise cellular function and underlying molecular mechanisms are uncharacterized. Methods: We first defined E70–E80 as the critical fetal period for the initial formation of rump or tail adipose tissue in sheep. To delineate the functional role of PDGFD, we performed knockdown experiments in ovine adipose-derived stem cells (ADSCs) and conducted transcriptomic profiling. Results: PDGFD knockdown significantly inhibited ADSCs proliferation and concurrently upregulated key adipogenic markers (PPARγ, FABP4). Transcriptomics revealed that this phenotype was mediated primarily through the profound downregulation of the chemokine CXCL8. Pathway analysis demonstrated that the PDGFD-CXCL8 axis co-regulates adipose plasticity by modulating both the PI3K-Akt and MAPK signaling pathways. Conclusion: We conclude that PDGFD is a master regulator of adipose tissue plasticity, driving progenitor expansion via a CXCL8-dependent mechanism. This PDGFD-CXCL8 regulatory axis orchestrates the cellular groundwork essential for the extensive fat deposition that defines the ovine fat-tail phenotype. Copyright

adipose tissue , ADSCs , CXCL8 , PDGFD , PI3K/MAPK signaling

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College of Animal Science and Technology, Shihezi University, Shihezi, China
Department of Zooengineering and Biotechnology, Kazakh National Agrarian Research University, Almaty, Kazakhstan
Xinjiang Uygur Autonomous Regional Animal Husbandry Station, Urumqi, China
State Key Laboratory of Sheep Genetic Improvement and Healthy Breeding, Institute of Animal Husbandry and Veterinary Sciences, Xinjiang Academy of Agricultural and Reclamation Sciences, Shihezi, China

College of Animal Science and Technology
Department of Zooengineering and Biotechnology
Xinjiang Uygur Autonomous Regional Animal Husbandry Station
State Key Laboratory of Sheep Genetic Improvement and Healthy Breeding

10 лет помогаем публиковать статьи Международный издатель

Книга Публикация научной статьи Волощук 2026 Book Publication of a scientific article 2026