The importance of quality control of lsdv live attenuated vaccines for its safe application in the field
Haegeman A. De Leeuw I. Saduakassova M. Van Campe W. Aerts L. Philips W. Sultanov A. Mostin L. De Clercq K.
September 2021MDPI
Vaccines
2021#9Issue 9
Vaccination is an effective approach to prevent, control and eradicate diseases, including lumpy skin disease (LSD). One of the measures to address farmer hesitation to vaccinate is guaranteeing the quality of vaccine batches. The purpose of this study was to demonstrate the importance of a quality procedure via the evaluation of the LSD vaccine, Lumpivax (Kevevapi). The initial PCR screening revealed the presence of wild type LSD virus (LSDV) and goatpox virus (GTPV), in addition to vaccine LSDV. New phylogenetic PCRs were developed to characterize in detail the genomic content and a vaccination/challenge trial was conducted to evaluate the impact on efficacy and diagnostics. The characterization confirmed the presence of LSDV wild-, vaccine-and GTPV-like sequences in the vaccine vial and also in samples taken from the vaccinated animals. The analysis was also suggestive for the presence of GTPV-LSDV (vaccine/wild) recombinants. In addition, the LSDV status of some of the animal samples was greatly influenced by the differentiating real-PCR used and could result in misinterpretation. Although the vaccine was clinically protective, the viral genomic content of the vaccine (being it multiple Capripox viruses and/or recombinants) and the impact on the diagnostics casts serious doubts of its use in the field.
Lumpy skin disease , Quality control , Recombinant , Vaccine
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Unit of Exotic and Particular Diseases, Scientific Directorate Infectious Diseases in Animals, Sciensano, Groeselenberg 99, Brussels, B-1180, Belgium
Kazakh Scientific Research Veterinary Institute (KazSRVI//KazNIVI), Raiymbek ave. 223, Almaty, 050016, Kazakhstan
Experimental Center Machelen, Scientific Directorate Infectious Diseases in Animals, Sciensano, Kerklaan 68, Machelen, B-1830, Belgium
EURL for Diseases Caused by Capripox Viruses, Scientific Directorate Infectious Diseases in Animals, Sciensano, Groeselenberg 99, Brussels, B-1180, Belgium
Unit of Exotic and Particular Diseases
Kazakh Scientific Research Veterinary Institute (KazSRVI//KazNIVI)
Experimental Center Machelen
EURL for Diseases Caused by Capripox Viruses
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