Portable biosensors based on the CRISPR/Cas system for detection of pathogen bacteria: Up-to-date technology and future prospects

Gupta J. Saquib abullais S. Althomali R.H. Margoth Guanga Chunata D. Shukhratovich Abdullaev S. Yeslam H.E. Sarsembenova O. Ramadan M.F. Alsalamy A. Alkhayyat S.
November 2023 Elsevier Inc.

Microchemical Journal
2023 #194

Up to now, the role of infectious pathogens bacteria in terms of severe human illnesses or even great deaths is undeniable. Over the last few years, the modern lifestyle has raised the problem of infectious pathogen bacteria more and more. Indeed, the rapid, sensitive and selective sensing platforms can prevent the spread of these microorganisms, additionally, optimize medical healthcare systems. In favor of this matter, clustered regularly interspaced short palindromic repeats (CRISPR)/Cas system, as a microbial defense system, has attracted considerable attention. To elaborate, although the system protects bacteria from being attacked by invading types, there are various types of developed biosensors using the CRISPR/Cas system for the determination of numerous targets, including bacteria. In addition, the progress of nanotechnology brings about more efficient probes and sensing approaches in terms of point-of-care (POC) detection. In favor of probes, the integration of different nanomaterials can improve stability, accuracy and sensitivity. In addition, using smartphones, microfluidics systems and lateral flow assays (LFAs) can provide portable analytical approaches for bacteria. With this regard, in this study, we aimed to comprehensively analysis current advances in portable biosensors based on the CRISPR/Cas systems and their exploitation in the quantification of pathogenic bacteria. In the first part of this study, the working principles of CRISPR/Cas systems for introducing high-potential biosensors were discussed. Afterwards, attention focused on the application of portable biosensors based on the CRISPR/Cas systems in pathogenic biosafety analysis. Eventually, the challenges and opportunities for the further progress of these types of sensing approaches were discussed.

CRISPR/Cas system , Lateral flow assay , Microfluidics system , Pathogen bacteria , Smartphones-based biosensors

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Institute of Pharmaceutical Research, GLA University, Pin Code 281406, U. P., Mathura, India
Department of Periodontics and Community Dental Sciences, College of Dentistry, King Khalid University, KSA, Abha, Saudi Arabia
Department of Chemistry, Prince Sattam Bin Abdulaziz University, College of Arts and Science, Wadi Al-Dawasir 11991, Saudi Arabia
Universidad Estatal de Bolívar, Facultad de Ciencias Agropecuarias, Recursos Naturales y del Ambiente, CP:020150, Guaranda, Ecuador
Faculty of Chemical Engineering, New Uzbekistan University, Tashkent, Uzbekistan
Scientific and Innovation Department, Tashkent State Pedagogical University Named After Nizami, Tashkent, Uzbekistan
CEO of the Company of “Editory” LLC
Department of Restorative Dentistry, King Abdulaziz University, Jeddah, Saudi Arabia
Department of Engineering Ecology and Occupational Safety, Gumarbek Daukeev, Almaty University of Energy and Communication, Almaty, 050000, Kazakhstan
College of Dentistry, Al-Ayen University, Thi-Qar, Iraq
College of Technical Engineering, Imam Jaafar Al‐Sadiq University, Al‐Muthanna, 66002, Iraq
College of Pharmacy, the Islamic University, Najaf, 54001, Iraq

Institute of Pharmaceutical Research
Department of Periodontics and Community Dental Sciences
Department of Chemistry
Universidad Estatal de Bolívar
Faculty of Chemical Engineering
Scientific and Innovation Department
CEO of the Company of “Editory” LLC
Department of Restorative Dentistry
Department of Engineering Ecology and Occupational Safety
College of Dentistry
College of Technical Engineering
College of Pharmacy

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