Gingival crevicular fluid levels of cytokine, chemokine, and growth factors in patients with periodontitis or gingivitis and periodontally healthy subjects: a cross-sectional multiplex study
Gündoǧar H. Üstün K. Senyurt S.Z. Özdemir E.Ç. Sezer U. Erciyas K.
2021Termedia Publishing House Ltd.
Central European Journal of Immunology
2021#46Issue 4474 - 480 pp.
Introduction: Gingival crevicular fluid (GCF) is an important resource for detecting inflammatory biomarkers related to periodontal disease. The purpose of this research was to identify the possible relation between cytokine levels and periodontal status. Material and methods: GCF samples collected from 25 periodontally healthy individuals, 24 with gingivitis, and 24 with periodontitis were analyzed using the MAGPIX system with a Bio-Plex Pro Human Cytokine 27-plex kit. Gingival index (GI), periodontal pocket depth (PPD), plaque index (PI), and clinical attachment level (CAL) were measured from each tooth to determine each patients periodontal status. Results: All clinical parameters showed statistically significant differences between groups. While interleukin (IL)-1β, IL-6, macrophage chemotactic protein 1 (MCP-1), interferon g-induced protein 10 (IP-10) and vascular endothelial growth factor (VEGF) levels were statistically significantly higher in patients with periodontitis compared to periodontally healthy subjects, IL-15 levels were found to be statistically significantly higher in periodontally healthy individuals compared to periodontitis (p < 0.05). Also, IL-1β and IP-10 showed positive correlations with PPD, CAL, GI and bleeding on probing (BOP). Conclusions: It is considered that IL-1β, IL-6, IL-15, MCP-1, VEGF and IP-10 could be related to periodontal disease and health and can be used as an adjunct to clinical examination in future research design. Scanning cytokines in GCF with a multiplex immunoassay technique is consequential, especially because many cytokine types are found in one sample.
Gingivitis , Laser doppler flow cytometry , Periodontal health , Periodontitis
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Department of Surgery and Pediatric Dentistry, Khoja Akhmet Yassawi International Kazakh-Turkish University, Turkestan, Kazakhstan
Department of Medical Biochemistry, Faculty of Medicine, University of Gaziantep, Gaziantep, Turkey
Department of Periodontology, Faculty of Dentistry, Gaziantep University, Turkey
Department of Periodontology, Faculty of Dentistry, Akdeniz University, Turkey
Department of Periodontology, Faculty of Dentistry, Kahramanmaras Sütçü Imam University, Turkey
Department of Periodontology, Faculty of Dentistry, Istanbul Gelisim University, Turkey
Department of Surgery and Pediatric Dentistry
Department of Medical Biochemistry
Department of Periodontology
Department of Periodontology
Department of Periodontology
Department of Periodontology
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