PROSPECTS FOR THE DEVELOPMENT OF TECHNOLOGY OF ADHESIVE FILMS WITH ASIAN MINT (MENTHA ASIATICA BORISS.) EXTRACT
Gazizova A. Datkhayev U. Amirkhanova A. Ustenova G. Kozhanova K. Kapsalyamova E. Kadyrbayeva G. Allambergenova Z. Kantureyeva A. Tastambek K. Baidullayeva A. Mombekov S.
January 2025Georgian Association of Business Press
Georgian Medical News
2025#367Issue 10195 - 211 pp.
The results of the development, standardization and biological evaluation of an innovative two-layer mucoadhesive dental film containing Mentha asiatica Boriss. extract and lidocaine hydrochloride for the treatment of inflammatory diseases of the oral cavity are presented. Materials and methods. Ethanol extract of Mentha asiatica Boriss. was obtained by Soxhlet extraction followed by freeze-drying. The chemical composition was determined by GC-MS, which revealed 1,8-cineole oxide and piperitenone. The analytical method demonstrated high linearity (R2 > 0.995), sensitivity (LOD = 0.01 μg/ml; LOQ = 0.05 μg/ml) and accuracy (RSD < 3%). Results. The optimized film (10% collagen, 10% gelatin, 2% glycerin) showed high elasticity, strength and adhesion. Physicochemical characteristics included: pH 6.5 ± 0.2, swelling time 3–5 min, deviation from homogeneity < 7% and complete dissolution in 10–16 min. The in vitro release profile of lidocaine hydrochloride demonstrated an initial rapid release (36.0% within 1 hour), followed by a sustained release reaching 95.0% at 24 hours, confirming the film’s ability to provide prolonged local analgesia. Microbiological purity complied with the standards of the Republic of Kazakhstan and the EAEU. Sterilization with ethylene oxide ensured a shelf life of more than 2 years. At a concentration of 31.25 μg/ml, the extract reduced NO production by RAW264.7 macrophages by 31.8%. SAEE exhibited selective cytotoxicity towards cancer cells while maintaining the safety of HUVEC. Compared to analogues (RU2739260C1, RU2734249C2), the resulting film was distinguished by better solubility, increased adhesion, and a unique composition. Conclusion. The developed two-layer mucoadhesive film combines antimicrobial, anti-inflammatory and analgesic effects, has improved physicochemical and biological properties, and can be considered a promising form for industrial production and clinical trials.
antimicrobial activity , asian mint , bioactive compounds , controlled release , dental films , extraction process , industrial production , Mentha asiatica Boriss , pharmaceutical formulation , quality standardization , Technology transfer
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School of Pharmacy, S.D. Asfendiyarov Kazakh National Medical University, Tole-bi 94, Almaty, 050012, Kazakhstan
Ecology Research Institute, Khoja Akhmet Yassawi International Kazakh-Turkish University, Turkestan, 161200, Kazakhstan
Sustainability of Ecology and Bioresources Institute, Al-Farabi Kazakh National University, Al-Farabi ave. 71, Almaty, 050040, Kazakhstan
School of Pharmacy
Ecology Research Institute
Sustainability of Ecology and Bioresources Institute
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