Antiparasitic activity of Colombian Amazon palm extracts against Giardia lamblia trophozoites: insights into cellular death mechanisms
García-Bustos J.J. Luna Pizarro G. Patolsky R.G. Joray M.B. Villalba-Vizcaino V. Galeano P. Espitia-Almeida F. Correa Múnera M. Ozturk M. Rópolo A.S. Feliziani C. Touz M.C. Laiolo J.
2025Frontiers Media SA
Frontiers in Microbiology
2025#16
Introduction: Colombian plants have a long history of use in traditional medicine and ethnopharmacology, particularly for treating stomach pain, digestive issues, diarrhea, and other gastrointestinal disorders. Recent studies have renewed interest in their potential therapeutic properties. Methods: This study evaluated the giardicidal activity of 15 crude plant extracts native to the Colombian Amazon against Giardia lamblia (genotype A, strain WB/1267). The MTT colorimetric assay was used to determine the effectiveness of these extracts at a concentration of 500 μg/mL. Extracts showing significant activity were further analyzed to determine their half-maximal inhibitory concentration (IC50). The cell death mechanisms of Attalea butyracea were studied using flow cytometry, confocal microscopy, and transmission electron microscopy (TEM). Results: Among the tested extracts, the Attalea butyracea fruit extract (P-2) exhibited the highest activity against WB/1267 (IC50 = 62.10 ± 6.57 μg/mL) and demonstrated giardicidal activity against GS/M (IC50 = 100.90 ± 3.40 μg/mL, genotype B) human infecting strains. These results prompted a detailed investigation into its mechanism of action using the WB/1267 strain as a model. At its IC50 concentration, P-2 primarily exerted its antiproliferative effect by induction of early apoptosis. A notable increase in late apoptosis and necrosis was observed at 2xIC50. Immunofluorescence assay (IFA) and confocal microscopy revealed chromatin condensation in treated trophozoites, while flow cytometry indicated G1/S cell cycle arrest. Furthermore, exposure to P-2 led to oxidative stress, evidenced by a significant increase in reactive oxygen species (ROS). The extract’s ability to disrupt various structural components of the parasite was confirmed through IFA and transmission electron microscopy. Interestingly, the P-2 extract effectively synergized with the first-line drug metronidazole against Giardia WB/1267 trophozoites. Discussion: These findings underscore the therapeutic potential of Colombian plant extracts in treating giardiasis, particularly highlighting the novel giardicidal activity of Attalea butyracea fruit extract and its promise for further therapeutic development. Copyright
Colombian Amazon , Giardia lamblia , giardicidal activity , medicinal plants , plant extracts
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Programa de Medicina Veterinaria y Zootecnia, Universidad de La Amazonia, Florencia, Caquetá, Colombia
Universidad del Magdalena, Facultad Ciencias de la Salud, Doctorado en Medicina Tropical SUE-Caribe, Grupo de Investigación en Inmunología y Patologia (GIPAT), Santa Marta, Colombia
Instituto de Investigación Médica Mercedes y Martín Ferreyra, Consejo Nacional de Investigaciones Científicas y Técnicas (INIMEC-CONICET), Universidad Nacional de Córdoba, Córdoba, Argentina
Centro de Investigación y Desarrollo en Inmunología y Enfermedades Infecciosas, Consejo Nacional de Investigaciones Científicas y Técnicas (CIDIE-CONICET-UCC), Universidad Católica de Córdoba, Córdoba, Argentina
Facultad de Ciencias Básicas, Universidad de La Amazonia, Florencia, Caquetá, Colombia
Centro de Investigaciones en Ciencias de la Vida, Facultad de Ciencias Básicas y Biomédicas, Universidad Simón Bolívar, Barranquilla, Colombia
Facultad de Ciencias Básicas, Programa de Biología, Universidad del Atlántico, Puerto Colombia, Colombia
Department of Chemistry, Faculty of Science, Mugla Sitki Koçman University, Mugla, Turkey
Faculty of Chemistry and Chemical Technology, Al-Farabi Kazakh National University, Almaty, Kazakhstan
Facultad de Ciencias de la Salud, Universidad Católica de Córdoba, Córdoba, Argentina
Programa de Medicina Veterinaria y Zootecnia
Universidad del Magdalena
Instituto de Investigación Médica Mercedes y Martín Ferreyra
Centro de Investigación y Desarrollo en Inmunología y Enfermedades Infecciosas
Facultad de Ciencias Básicas
Centro de Investigaciones en Ciencias de la Vida
Facultad de Ciencias Básicas
Department of Chemistry
Faculty of Chemistry and Chemical Technology
Facultad de Ciencias de la Salud
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