Multilocus DNA polymorphism of some rubber-bearing dandelions (Taraxacum spp.) of Russia and Kazakhstan
Gabit B. Karina G. Gulnar G. Kairat U. Yaroslav G. Aleksey C. Bulat K.
January 2022Springer Science and Business Media B.V.
Genetic Resources and Crop Evolution
2022#69Issue 1335 - 348 pp.
Successful domestication and introduction into culture of new forms of rubber-bearing dandelions require not only determination of the content and quality of rubber in them, but also accurate species identification and genetic certification of valuable lines and forms. The objects of our study were three samples of Taraxacum kok-saghyz Rodin KAZ08-023, L2, Sary Jaz, brown-seeded and pink-seeded forms of Taraxacum hybernum Stev., as well as along one sample of Taraxacum brevicorniculatum Korol., Taraxacum glaucanthum (Ledeb.) DC., and Taraxacum officinale L. The aim of our work was to search of RAPD, ISSR, and SSR markers for the unambiguous identification of all studied dandelion forms. The RAPD primers OPD-07 and FS-10 are best suited for the detection of interspecific polymorphism, while the RAPD primers FS-10, OPC-05 and OPC-08 can be used for the detection of intraspecific dandelion polymorphism. The ISSR primers HB10, ISSR17, and ISSR36 were most effective for detecting interspecific polymorphism. For identification of the brown-seeded and pink-seeded forms of T. hybernum, primers HB10 and ISSR38 can be used. Primers HB10 and ISSR14 are best suited for identifying the three studied kok-saghyz samples. SSR primers TKS0091, TKS0097, TKS0107, and TKS0110 are suitable for SSR identification of pink-seeded and brown-seeded forms of T. hybernum. Only locus TKS0111 is suitable for unambiguous SSR identification of all three studied kok-saghyz samples.
ISSR , RAPD , SSR , Taraxacum brevicorniculatum , Taraxacum glaucanthum , Taraxacum hybernum , Taraxacum kok-saghyz
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Kazakh National Agrarian Research University, Almaty, Kazakhstan
Department of Cell Engineering, Institute of Plant Biology and Biotechnology, Ministry of Education and Science of Republic of Kazakhstan, Almaty, Kazakhstan
Federal Research Centre of RAS, Institute of Biochemistry and Genetics of Ufa, Ufa, Russian Federation
Ufa Federal Research Centre, South-Ural Botanical Garden-Institute, Russian Academy of Sciences, Ufa, Russian Federation
Kazakh National Agrarian Research University
Department of Cell Engineering
Federal Research Centre of RAS
Ufa Federal Research Centre
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