Bioremediation of Oil-Contaminated Soil of the Republic of Kazakhstan Using a New Biopreparation


Funtikova T.V. Akhmetov L.I. Puntus I.F. Mikhailov P.A. Appazov N.O. Narmanova R.A. Filonov A.E. Solyanikova I.P.
February 2023MDPI

Microorganisms
2023#11Issue 2

A new biopreparation is developed to clean soils from oil pollution in the arid climate of the Republic of Kazakhstan. The biopreparation includes bacterial strains R. qingshengii F2-1, R. qingshengii F2-2, and P. alloputida BS3701. When using the biopreparation in a liquid mineral medium with 15% crude oil, laboratory studies have revealed degradation of 48% n-alkanes and 39% of PAHs after 50 days. The effectiveness of the biopreparation has been demonstrated in field experiments in the soil contaminated with 10% crude oil at the K-Kurylys landfill, Republic of Kazakhstan. During the six-month field experiment, the number of oil degraders reached 107 CFU/g soil, which degraded 70% of crude oil by the end of the experiment.

alkanes , biodegradation , crude oil , field trials , microbial consortia , PAHs

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Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences, G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms of the Russian Academy of Sciences, Prospect Nauki 5, Pushchino, 142290, Russian Federation
Department of Ecology and Chemical Technology, Korkyt Ata Kyzylorda University, Aiteke Bi Str. 29A, Kyzylorda, 120000, Kazakhstan

Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences
Department of Ecology and Chemical Technology

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