Mechanism of the antimicrobial activity induced by phosphatase inhibitor sodium ortho-vanadate
Fan H. Dukenbayev K. Nurtay L. Nazir F. Daniyeva N. Pham T.T. Benassi E.
September 2024Elsevier Inc.
Journal of Inorganic Biochemistry
2024#258
The present study describes a novel antimicrobial mechanism based on Sodium Orthovanadate (SOV), an alkaline phosphatase inhibitor. Scanning electron microscopy (SEM), transmission electron microscopy (TEM) and atomic force microscopy (AFM) were employed to examine the surface morphologies of the test organism, Escherichia coli (E. coli), during various antibacterial phases. Our results indicated that SOV kills bacteria by attacking cell wall growth and development, leaving E. colis outer membrane intact. Our antimicrobial test indicated that the MIC of SOV for both E. coli and Lactococcus lactis (L. lactis) is 40 μM. A combination of quantum mechanical calculations and vibrational spectroscopy revealed that divanadate from SOV strongly coordinates with Ca2+ and Mg2+, which are the activity centers for the phosphatase that regulates bacterial cell wall synthesis. The current study is the first to propose the antibacterial mechanism caused by SOV attacking cell wall.
Alkaline phosphatase inhibitor , Antimicrobial , Quantum mechanical calculations , Sodium orthovanadate , Vibrational spectroscopy
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Department of Chemistry, School of Sciences and Humanities, Nazarbayev University, Nur-Sultan, 010000, Kazakhstan
Department of Electrical and Computer Engineering, School of Engineering and Digital Sciences, Nazarbayev University, Nur-Sultan, 010000, Kazakhstan
Department of Biology, School of Sciences and Humanities, Nazarbayev University, Nur-Sultan, 010000, Kazakhstan
Core Facility, Nazarbayev University, Nur-Sultan, 010000, Kazakhstan
Novosibirsk State University, Pirogov str. 2, Novosibirsk, 630090, Russian Federation
Department of Chemistry
Department of Electrical and Computer Engineering
Department of Biology
Core Facility
Novosibirsk State University
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