Carbon nanoparticles induce DNA repair and PARP inhibitor resistance associated with nanozyme activity in cancer cells
Fan H. Sun Q. Dukenbayev K. Benassi E. Manarbek L. Nurkesh A.A. Khamijan M. Mu C. Li G. Razbekova M. Chen Z. Amin A. Xie Y.
December 2022BioMed Central Ltd
Cancer Nanotechnology
2022#13Issue 1
Background: Quantum nanodots especially carbon nanoparticles (CNPs) have been widely studied in biomedicine in imaging, and drug delivery, but anti-cancer mechanisms remain elusive. Methods: Here, we investigated a type of cell death induced by food (beet, soybean) derived CNPs in cancer cells and tested whether CNPs induced DNA damage and resistant to anti-cancer agent PARP inhibitor (PARPi) could be overcome by quantum calculations, TEM, AFM, FT-IR, soft agar assay, and cytotoxicity assay. Results: At high doses, CNPs derived from beet lead to a pop-like apoptosis (Carbopoptosis) in cancer cells. Quantum mechanical calculations confirmed CNPs binding with phosphate groups as well as DNA bases. At low doses, CNPs develop PARPi drug resistance through interactions between CNPs and PARPi. A synergistic drug effect was achieved with the combination of phosphatase inhibitor (PPi), PARPi, and CNPs. This is corroborated by the fact that sulfur modulated CNPs which exhibit super high phosphatase nanozyme activity abrogated the CNPs induced colony formation in anchorage-independent cancer cell growth. Conclusion: Thus, our data suggest the CNPs intrinsic nanozyme activity of phosphatase may crosstalk with drug resistance, which can be reversed upon modulations.
Carbon nanoparticles , Carbopoptosis , Drug resistance , PARP inhibitor
Text of the article Перейти на текст статьи
Department of Chemistry, School of Sciences and Humanities, Nazarbayev University, Astana, 010000, Kazakhstan
Key Laboratory for Applied Technology of Sophisticated Analytical Instrument of Shandong Province, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan, China
Department of Electrical and Computer Engineering, School of Engineering and Digital Science, Nazarbayev University, Astana, 010000, Kazakhstan
Faculty of Natural Sciences, Novosibirsk State University, Novosibirsk, Russian Federation
Department of Biology, School of Sciences and Humanities, Nazarbayev University, Astana, 010000, Kazakhstan
Zhejiang University-Hangzhou Global Scientific and Technological Innovation Center, Hangzhou, China
Department of Biochemistry, Cancer Biology, Neuroscience and Pharmacology, Meharry Medical College, Nashville, 37208, TN, United States
Biology Department, UAE University, Al Ain, 15551, United Arab Emirates
Department of Chemistry
Key Laboratory for Applied Technology of Sophisticated Analytical Instrument of Shandong Province
Department of Electrical and Computer Engineering
Faculty of Natural Sciences
Department of Biology
Zhejiang University-Hangzhou Global Scientific and Technological Innovation Center
Department of Biochemistry
Biology Department
10 лет помогаем публиковать статьи Международный издатель
Книга Публикация научной статьи Волощук 2026 Book Publication of a scientific article 2026