Epitopes specificity of antibodies to thyroid peroxidase in patients with Graves’ disease, Hashimotos thyroiditis and overlap-syndrome
Espenbetova M. Kuzmina N. Zubkov A. Akhmetova V. Zamanbekova Z. Krykpaeva A. Zhumanbayeva Z. Amrenova K. Smailova Z. Glushkova N.
March 2022Elsevier B.V.
Journal of Clinical and Translational Endocrinology
2022#27
Background: Antibodies against thyroid peroxidase (anti-TPO) serve as clinical markers of thyroid autoimmune diseases (TAIDs). By trying to elucidate the causes of heterogeneity in autoantibody levels among patients with different TAIDs it becomes possible to clarify the pathophysiology of GD and HT. Objective: To investigate the heterogeneity of epitopes recognized by anti-TPO in patients with Hashimotos thyroiditis (HT), Graves’ disease (GD) and overlap-syndrome. Methods: We carried out a cross-sectional study on 398 patients with GD, HT and overlap syndrome and analyzed the specificity of epitopes and binding constants of TPO with monoclonal antibodies (MAbs). Ten MAbs to TPO were used, of which five were reactive with native TPO and the rest were reactive with denaturated TPO. Results: The autoantibodies in blood serum of HT patients inhibited the binding of MAb63 more significantly than those in serum of GD patients: 59.62 % versus 54.02 %, respectively (p = 0.001). The anti-TPOs in serum of GD patients inhibited the binding of MAb77 more significantly than those in serum of HT patients: 54.36 % versus 51.13 %, respectively (p = 0.047). The binding of MAb45 was more inhibited in serum of patients with anti-TPO concentration over 1000 IU/ml (58.36 %). The blood serum of patients with overlap-syndrome showed less significant inhibition of MAb63 binding than that of patients with no overlap-syndrome: 52.47 % versus 58.81 %, respectively (p = 0.043). Conclusion: Mapping the epitopes to TPO with the help of MAbs may improve the differential diagnosis between different thyroid autoimmunities.
Epitopes , Graves’ disease , Hashimotos thyroiditis , Thyroid autoimmune disorders , Thyroid peroxidase
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Department of Endocrinology, Semey Medical University, Semey, Kazakhstan
Testing Laboratory for Evaluation of Medicinal Products’ Quality, I.I. Mechnikov Research Institute, Moscow, Russian Federation
Research Laboratory, Department of Immunology, I.I. Mechnikov Research Institute, Moscow, Russian Federation
Research Department, Semey Medical University, Semey, Kazakhstan
Department of Nursing, Semey Medical University, Semey, Kazakhstan
Department of Propaedeutics of Internal Diseases, Semey Medical University, Semey, Kazakhstan
Department of Biochemistry and Chemical Disciplines Named After Doctor of Medical Sciences, Semey Medical University, Semey, Kazakhstan
Department of Epidemiology, Biostatistics & Evidence Based Medicine, Al-Farabi Kazakh National University, Almaty, Kazakhstan
Department of Endocrinology
Testing Laboratory for Evaluation of Medicinal Products’ Quality
Research Laboratory
Research Department
Department of Nursing
Department of Propaedeutics of Internal Diseases
Department of Biochemistry and Chemical Disciplines Named After Doctor of Medical Sciences
Department of Epidemiology
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