Amplification-free detection of Ascochyta blight in chickpea using a simple molecular beacon assay
Dyussembayev K. Akpe V. Yechshzhanov T. Cheesman M.J. Kim T.H. Cock I.E.
December 2024Nature Research
Scientific Reports
2024#14Issue 1
Ascochyta blight is a major biotic stress that limits chickpea production globally. Fungicide application remains one of the effective control measures for the endemic spread. Due to the serious threat that synthetic fungicides pose to crop quality, early diagnosis of the pathogen is imperative. Whilst there have previously been several conventional lab-based diagnostic methods developed for early detection of Ascochyta rabiei, they require long assay times, specialised equipment and facilities, and trained personnel to process the samples. To overcome this challenge, a rapid amplification-free detection assay using a molecular beacon probe was developed. The method consists of a simple assembly assay that accurately detects pathogen within 30 min. The developed assay is species-specific and has a similar sensitivity level as conventional amplification-based methods. Although it is still a lab-based technique, considering the simplicity of the assay, it has a great potential to be developed further as a reliable in-field diagnostic device for early detection and quantification of fungal pathogen spores.
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School of Environment and Science, Griffith University, Nathan, 4111, QLD, Australia
L.N. Gumilyov Eurasian National University, Astana, 010000, Kazakhstan
Pedagogical Institute, Astana International University, Astana, 010000, Kazakhstan
Menzies Institute, Griffith University, Nathan, 4111, QLD, Australia
School of Pharmacy and Medical Sciences, Griffith University, Gold Coast, 4222, QLD, Australia
Queensland Micro and Nanotechnology Centre, Griffith University, Nathan, 4111, QLD, Australia
Centre for Planetary Health and Food Security, Griffith University, Nathan, 4111, QLD, Australia
School of Environment and Science
L.N. Gumilyov Eurasian National University
Pedagogical Institute
Menzies Institute
School of Pharmacy and Medical Sciences
Queensland Micro and Nanotechnology Centre
Centre for Planetary Health and Food Security
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